实用医学杂志2017，Vol.33Issue(1)：59-63,5.DOI:10.3969/j.issn.1006-5725.2017.01.016
1，25（OH）2D3通过抑制STAT3活化改善博来霉素诱导的小鼠肺纤维化
1,25(OH)2D3 protects against BLM-induced pulmonary fibrosis by inhibiting activation of STAT3
摘要
Abstract
Objective To investigate the impact of 1,25(OH)2D3 on histological changes and activation of STAT3 in BLM⁃induced pulmonary fibrosis mice. Methods 30 male C57BL/6 mice were randomly divided into control group ,BLM group and BLM+VD group. Mice in BLM group and BLM+VD group received intratracheal injection of BLM(3 U/kg). Control group were intratracheally injected equal volume of sterile saline. From the first day after the surgery,mice in BLM+VD group received intraperitoneal injection of VD (5μg/kg·d). After 21 days, H&E and Masson′s trichrome staining were carried out. Aschroft score were used to evaluate histological changes in lungs. IL⁃6,IL⁃4 and INF⁃γin BALF were assessed by Elisa. p⁃STAT3,α⁃SMA and Collagen I were detected by western blot (WB) and immunohistochemistry. Results Fibrosis score and level of α⁃SMA,Collagen I in BLM group were significantly higher than that in control group (P < 0.05). However ,treatment with VD effectively at⁃tenuated fibrosis (P<0.05). IL⁃6 and IL⁃4 increased while INF⁃γwas decreased in BALF of BLM group (P<0.05). VD could ameliorate these changes. Upregulation and neuclear translocation of p⁃STAT3 were observed in BLM group,while VD intervention could inhibit phosphorylation of STAT3. Conclusions VD attenuate BLM⁃induced pulmonary fibrosis and regulate inflammatory cytokines probably by blocking STAT3 activation.关键词
肺纤维化/1,25(OH)2D3/STAT3/博来霉素Key words
Pulmonary fibrosis/1,25(OH)2D3/STAT3/Bleomycin引用本文复制引用
罗丽珊,董航明,黄超文,胡亚惠,余常辉,万轩,赵海金,蔡绍曦..1，25（OH）2D3通过抑制STAT3活化改善博来霉素诱导的小鼠肺纤维化[J].实用医学杂志,2017,33(1):59-63,5.基金项目
国家自然科学基金（编号81470228、81270087、81670026）；国家重点研发计划 （）
