实用医学杂志2017，Vol.33Issue(2)：202-205,4.DOI:10.3969/j.issn.1006-5725.2017.02.010
EGFR抑制剂AG1478对宫颈癌HeLa细胞凋亡的影响
Effect of epidermal growth factor receptor inhibitor AG1478 induces cell death in HeLa cells
马聪玲 1殷一萍 1毛婷婷1
作者信息
- 1. 310051 浙江省杭州市武警医院药剂科
- 折叠
摘要
Abstract
Objective To investigate the biological features of epidermal growth factor receptor inhibitor AG1478 on cervical carcinoma cell. Methods The proliferation of HeLa cells under AG1478 stimulation was determined by CCK8 assay. The expression of EGFR downstream signaling protein and apoptotic relative protein were examined by Western blot and transcription of apotosis⁃related genes were measured by RT⁃qPCR in AG 1478 treated HeLa cells. Nuclear transport of phosphorylated ERK were measured through ICC assay. TUNEL assay was used to determine early stage of apoptosis. Results CCK8 assay showed that AG1478 inhibit the proliferation of HeLa cells and also block phosphorylated level of EGFR ,ERK and AKT. Furthermore ,nuclear transport of phosphorylated ERK upon EGF stimulation were blocked and pro⁃apoptotic proteins were up⁃regluated with activat ed cleaved Caspases. Conclusion AG1478 inhibited the proliferation and induced apoptosis in HeLa cells and it could be a potential therapeutic drug for cervical carcinoma.关键词
宫颈癌细胞/AG1478/EGFR/磷酸化/凋亡Key words
Cervical carcinoma cell/AG1478/EGFR/Phosphorylation/Apoptosis引用本文复制引用
马聪玲,殷一萍,毛婷婷..EGFR抑制剂AG1478对宫颈癌HeLa细胞凋亡的影响[J].实用医学杂志,2017,33(2):202-205,4.
