深圳大学学报（理工版）2017，Vol.34Issue(1)：33-45,13.DOI:10.3724/SP.J.1249.2017.01033
肺腺癌和肺鳞癌中mRNA可变剪接特征的比较
Comparison of alternative splicing in lung adenocarcinoma and lung squamous carcinoma
摘要
Abstract
Lung cancer is a common disease with high morbidity and mortality.Studies have shown that alternative splicing(AS)is closely associated with lung carcinogenesis,but the difference of alternative splicing between lung adenocarcinoma(LUAD)and lung squamous carcinoma(LUSC)remains unclear.Here,we analyze the RNA-Seq data of LUAD and LUSC from TCGA using SUPPA software,and find that skipping exon(SE)is the most frequently occurred AS transcript among seven main AS transcripts identified.By comparing tumor tissues with normal ones,we systematically identify differentially expressed AS transcripts in both LUAD and LUSC,of which around 60％are identical between these two subtypes.Functional analysis reveals that tumor-specific AS transcripts are mainly enriched among genes associated with cell cycle regulation,deoxyribonucleic acid metabolism and RNA metabolism,whereas normal-specific AS transcripts are enriched among genes mainly involved in vesicle-mediated transport,peptide transport and lipid transport.By comparing AS transcripts of cancer-driven genes between LUAD and LUSC,we identify some common and subtype-specific AS transcripts with cancer-driven potentiality.关键词
生物信息学/肺腺癌/肺鳞癌/可变剪接/外显子跳跃/可变起始外显子Key words
bioinformatics/lung adenocarcinoma (LUAD)/lung squamous carcinoma (LUSC)/alternative splicing (AS)/skipping exon (SE)/alternative first exon (AF)分类
医药卫生引用本文复制引用
陈梦竹,王秀杰,夏立新..肺腺癌和肺鳞癌中mRNA可变剪接特征的比较[J].深圳大学学报（理工版）,2017,34(1):33-45,13.基金项目
National High-Tech Research and Development Program (2012AA020409) （2012AA020409）
Shenzhen Science and Technology Basic Research Foundation(JCYJ20150525092941055) 国家高技术研究发展计划资助项目(2012AA020409) （JCYJ20150525092941055）
深圳市知识创新计划基础研究资助项目(JCYJ20150525092941055) （JCYJ20150525092941055）
