天津中医药2017，Vol.34Issue(2)：89-93,5.DOI:10.11656/j.issn.1672-1519.2017.02.06
基于基因芯片技术的咳喘六味合剂治疗寒哮型支气管哮喘的靶点发现和机制研究
The target-finding and mechanism of drug action researching of Kechuan Liuwei Mixture treating asthma of cold type based on cDNA microarrays technology
摘要
Abstract
Objective] The aim of this study was to explore the target genes and mechanism of drug action of Kechuan Liuwei Mixture. [Methods] The 16 patients who were diagnosed with asthma of cold type were selected to do the gene chip screening. The 8 cases of them took Kechuan Liuwei Mixture three times a day, 20 mL per time, while the others took the placebo. Both groups were treated for one week. Peripheral blood samples were taken after one-week drug intervention. Total RNA was extracted from peripheral blood. Using gene expression times numerical 1.5 and-1.5 for threshold to determine the differential expression genes,then GO and pathway analysis were used to analyze the differential expression genes on function and classification. [Results] Screening and identification of differentially expressed genes, 233 1.5-fold DEGs were screened from the gene expression spectrum, while 106 genes were up-regulated and 127 genes were down-regulated. These differently expressed genes are involved in functions of inflammatory responses,immune response,Cytokine-cytokine receptor interaction,transforming growth factor and pathways of Cytokine-cytokine receptor interaction, Focal adhesion, Calcium signaling pathway, MAPK signaling pathway , ECM-receptor interaction of asthma. [Conclusion] Kechuan Liuwei Mixture could treat asthma of cold type through multi-channels and multi-targets by effecting genes that related to immune response, inflammatory reaction, airway remodeling and calcium channels.关键词
咳喘六味合剂/寒性哮喘/基因芯片/靶点基因/作用机制Key words
Kechuan Liuwei Mixture/asthma of cold type/cDNA microarrays technology/target gene/mechanism of drug action分类
医药卫生引用本文复制引用
邹璐,方泓,吴银根..基于基因芯片技术的咳喘六味合剂治疗寒哮型支气管哮喘的靶点发现和机制研究[J].天津中医药,2017,34(2):89-93,5.基金项目
上海市科委“科技创新行动计划”医学与农业领域项目。 （）
