现代妇产科进展2017，Vol.26Issue(2)：96-98,103,4.DOI:10.13283/j.cnki.xdfckjz.2017.02.004
MDM2基因启动子区40-bp插入/缺失多态性与宫颈癌易感性相关研究
An insertion/deletion polymorphism within the constitutive promoter of MDM2 associated with susceptibility for cervical cancer in the Chinese population
摘要
Abstract
Objective:To investigate the association between CC susceptibility with a 40-bp insertion/deletion polymorphism ( rs3730485 ) in the distal promoter of MDM2 . Method:The genotype of MDM2 was detected by RCR,then the PCR products were detected by 2. 5%agarose gel electrophoresis,EB colorimetry,product 521bp and 481bp two bands,according to the final product band location and quantity to determine the genotype of sample,the final count by two different subjects. Result:No significant differences were found between CC patients and control subjects in terms of age,there was prominent difference of the association smoking,and drinking status(P<0. 05),suggesting that the frequency matching was adequate. Compared with the ins/ins genotype,subjects with the heterozygous ins/del or homozygous del/del had a signif-icantly increased risk of CC (adjusted OR=1. 51,P=0. 009 and OR=1. 86,P=0. 01,respec-tively) . Conclusion:Our study provided initial evidence that the indel may play a functional role in development of CC in the Chinese population. Positive association was found in stratifica-tion analysis on smoking status and drinking status,while no significant difference was found on risk factors of age.关键词
MDM2基因/插入/缺失多态性/宫颈癌/rs3730485Key words
MDM2 gene/Ins/del/Cervical cancer/rs3730485分类
医药卫生引用本文复制引用
冯兰君,李朝阳,朱占胜,王会平,陆晓媛..MDM2基因启动子区40-bp插入/缺失多态性与宫颈癌易感性相关研究[J].现代妇产科进展,2017,26(2):96-98,103,4.基金项目
国家自然科学基金项目(No:81502428) （No:81502428）
江苏省自然科学基金项目(No:BK20140222,15KJB310024,BK20150220) （No:BK20140222,15KJB310024,BK20150220）
徐州市科技局立项支撑项目(No:KC14SH113) （No:KC14SH113）
