现代妇产科进展2017，Vol.26Issue(2)：99-103,5.DOI:10.13283/j.cnki.xdfckjz.2017.02.005
RNA干扰抑制EZH2基因表达对子宫内膜癌细胞上皮-间质转化的影响
Influence of inhibition of EZH2 gene expression by RNA interference on epithelial-mes-enchymal transition in endometrial carcinoma cells
摘要
Abstract
Objective:To explore the effects on cell proliferation and migration as well as epithelial-mesenchymal transition ( EMT) after suppressing EZH2 expression in endometrial carcinoma. Methods:RT-PCR and Western blot were used to examine the expression of EZH2 in normal endometrial cell line ESC and endometrial carcinoma cell lines ECC-1 and RL95-2 . SiRNAs targeting to EZH2 were transfected into endometrial carcinoma cells ECC-1 and RL95-2,CCK-8 and transwell migrating assay were utilized to examine the cell proliferation and mi-gration. Western blot was applied to investigate the protein expression of E- cadherin、α-cate-nin、N-cadherin and Vimentin after siRNA targeting to EZH2 in endometrial carcinoma cell lines ECC-1 and RL95-2 . Results:The expression of EZH2 in endometrial carcinoma cell lines ECC-1 and RL95-2 were higher than in normal endometrial cell line ESC ( P<0 . 01 ) . Further more,knocking down EZH2 expression could inhibit cell proliferation and migration in endome-trial carcinoma cell lines ECC-1 and RL95-2 ( P<0 . 01 ) . Suppression of EZH2 could upregulat-ed the expression of E-cadherin、α-catenin protein(P<0. 05) and downregulated the expression of N-cadherin and Vimentin protein ( P<0 . 01 ) . Conclusion:EZH2 expression is increased in endometrial carcinoma cell lines. Knocking down EZH2 expression suppresses the cell prolifera-tion,migration and epithelial-mesenchymal transition in endometrial carcinoma cells.关键词
子宫内膜癌/EZH2基因/增殖/迁移/上皮-间质转化Key words
Endometrial carcinoma/EZH2 gene/Proliferation/Migration/Epithelial-mesenchymal transition( EMT)分类
医药卫生引用本文复制引用
王娟,马莉,陈静,艾志宏,滕银成..RNA干扰抑制EZH2基因表达对子宫内膜癌细胞上皮-间质转化的影响[J].现代妇产科进展,2017,26(2):99-103,5.基金项目
上海市科委基金资助项目(No:20134226) （No:20134226）
