现代妇产科进展2017，Vol.26Issue(2)：124-128,5.DOI:10.13283/j.cnki.xdfckjz.2017.02.011
短串联重复序列STR分析在葡萄胎诊断中的作用研究
The application of STR ( short tandem repeat ) analysis in the differential diagnosis of Hydatidiform mole
摘要
Abstract
Objective:To explore the use of STR ( short tandem repeats ) analysis in the differential diagnostic of Hydatidiform Mole. Methods:40 cases of pathological complete hy-datidiform mole,20 cases of partial hydatidiform mole and 20 cases of hydropic abortus were collected from the urumqi Maternal and Child Health Hospital Department of Pathology from 2000 to 2015. Tissue samples were collected for STR analysis,which included seven specific lo-ci (D7S820,D13S317,D16S59,TPOX,TH01,CSF1PO,VWA). And then the value of differ-ential diagnosis for hydatidiform mole was further assessed. Results:Histopathologically,the hy-dropic abortus often revealed focal villous swelling. And the partial hydatidiform mole ( PHM) is characterized by focal trophoblastic hyperplasia and hydatidiform villous swelling. However, the complete hydatidiform mole ( CHM) usually showed obvious villous trophoblastic hyperpla-sia and villous swelling with pool development. In terms of STR analysis,the hydropic abortus showed balanced biallelic profiles of both paternal and maternal origins in the villous tissue (18/18). STR analysis failed to be generated in 2wo cases of hydropic abortus. Of the 20 PHM cases,2 cases were MPM ( monospermic partial hydatidiform mole) and 14 were DPM ( disper-mic partial hydatidiform mole,DPM) . Another 3 cases showed balanced biallelic profiles of both paternal and maternal origins (3/19) and the STR analysis failed to be generated in 1 case. Of the 40 CHM cases,25 cases were MCM(monospermic complete hydatidiform mole) and 10 were DCM ( dispermic complete hydatidiform mole,DCM) . The STR analysis failed to be gener-ated in the remaining 5 cases of CHM. Conclusion:The histopathologic diagnosis of partial hy-datidiform mole is still not so accurate. STR analysis including 7 sites have important role for the differential diagnosis of complete hydatidiform mole,partial hydatidiform mole and hydropic abortus.关键词
葡萄胎/短串联重复序列/鉴别诊断Key words
Mole/Immunohistochemistry/Short tandem repeats/Differential diagnosis分类
医药卫生引用本文复制引用
陈永峰,薛荣,刘璇,陈云新,沈丹华..短串联重复序列STR分析在葡萄胎诊断中的作用研究[J].现代妇产科进展,2017,26(2):124-128,5.基金项目
乌鲁木齐市科学技术局基金(No:Y141310055) （No:Y141310055）
