亚热带植物科学2016，Vol.45Issue(4)：308-314,7.DOI:10.3969/j.issn.1009-7791.2016.04.002
小白菊内酯及降温速率对含水生菜种子抗冷性的影响
Effects of Parthenolide and Decending Speed of Temperature on Chilling Resistance of Imbibed Seeds of Lactuca sativa
沈梦琪 1韩颖颖 1周强 1李维杰 1刘宝林1
作者信息
- 1. 上海理工大学医疗器械与食品学院，生物系统热科学研究所，上海 200082
- 折叠
摘要
Abstract
In order to study the effects of Parthenolide on chilling resistance of Lactuca sativa seeds during programmed cooling, the seeds were imbibed by exogenous animal ubiquitin E3 ligase activator Parthenolide before treated with programmed cooling with different cooling rates. The results showed that the addition of Parthenolide reduced the freezing tolerance of lettuce seeds in both fast and slow cooling treatments, and the germination rates after both cooling treatments were significantly lower than control, the effects of Parthenolide were the most obvious when the temperature was reduced to -20 ℃, which indicating that during the programmed cooling, the ubiquitination regulatory pathway did participate in the resistance to freezing in seeds. The expression of ubiquitination-related genes and ICE1 gene were detected by real-time quantitative PCR. It was found that Parthenolide inhibited the expression of RING-finger E3 ligase COP1 and anti-freezing factor ICE1 during the slow cooling. The effects of Parthenolide on the activities of SOD were further proved that during the programmed cooling process, Parthenolide may destroy the freezing resistance system of lettuce seeds.关键词
生菜/种子/程序降温/降温速率/低温冷冻胁迫/小白菊内酯Key words
Lactuca sativa/seed/programmed cooling/cooling rate/chilling stress/Parthenolide分类
农业科技引用本文复制引用
沈梦琪,韩颖颖,周强,李维杰,刘宝林..小白菊内酯及降温速率对含水生菜种子抗冷性的影响[J].亚热带植物科学,2016,45(4):308-314,7.
