中国美容医学2017，Vol.26Issue(1)：13-16,4.
低剂量ALA-PDT对人角质形成细胞生长和分泌细胞因子的作用研究
Effects of Low Dose ALA-PDT on the Cell Growth and Cytokine Secretion in Human Keratinocytes
摘要
Abstract
ObjectiveTo study effects of different doses of 5-aminolevulinic acid photodynamic (ALA-PDT) on the proliferation and secretion of growth factors in cultured keratinocytes.MethodsThe proliferation of HaCaT cell line and cultured human keratinocytes were detected by MTT. The contents of VEGF and bFGF in supernatant of cultured human keratinocytes were detected by ELISA.Results Low-dose PDT-ALA (0.1mM and 0.01mM) and 21J/cm2 red light-promoted the proliferation of HaCaT cells and human keratinocytes in vitro. High-dose ALA-PDT-0.5mM ALA and 21J/cm2 red light-inhibited the proliferation of HaCaT cells and human keratinocytes in vitro. After 24h treated by low-dose ALA-PDT, the secretion of bFGF in human keratinocytes was signiifcantly higher than that in 0.5mM ALA group and red light group. The secretion of VEGF in human keratinocytes treated by ALA-PDT showed little change within 24h. But it was obviously increased after 24h.ConclusionHigh-dose ALA-PDT inhibited the proliferation of keratinocytes. Low-dose ALA-PDT could promote the proliferation of keratinocytes, and improve the secretion of bFGF and VEGF.关键词
HaCaT细胞/角质形成细胞/光动力治疗/ALA-PDT/碱性成纤维细胞生长因子/血管内皮生长因子Key words
HaCaT/Keratinocyte/PDT/ALA-PDT/bFGF/VEGF分类
医药卫生引用本文复制引用
杨航,谭杨,唐辉,杨涛,杨桂红,雷霞..低剂量ALA-PDT对人角质形成细胞生长和分泌细胞因子的作用研究[J].中国美容医学,2017,26(1):13-16,4.基金项目
国家自然科学基金资助项目 （）
