中国药理学通报2017，Vol.33Issue(2)：171-174,4.DOI:10.3969/j.issn.1001-1978.2017.02.006
蛋白酪氨酸磷酸酶 SHP-2在Ⅰ型代谢型谷氨酸受体诱发痛觉超敏中的作用
Role of protein tyrosine phosphatase SHP-2 in group I metabotropic glutamate receptors-induced allodynia
摘要
Abstract
Aim To investigate whether the pain modi-fication by group I metabotropic glutamate receptors (mGluRs)required the involvement of Src homology-2 domain-containing phosphatase-2 (SHP-2 ).Methods Co-immunoprecipitation was performed to examine the possible interaction between SHP-2 and group I mGluRs in spinal cord dorsal horn of mice.By measur-ing the paw withdrawal thresholds,the effects of SHP-2 inhibitor NSC-87877 or its catalytically inactive SHP-2 (C459S ) mutant on allodynia induced by group I mGluRs agonist DHPG (50 nmol)were observed.Re-sults Anti-mGluR5 antibody was able to co-immuno-precipitate SHP-2 from spinal dorsal horn of mice, while no SHP-2 was precipitated by anti-mGluR1 anti-body.Inactivation of SHP-2 by NSC-87877 (6 nmol) or SHP-2 (C459S ) effectively attenuated allodynia caused by DHPG.Conclusion SHP-2 can physically interact with mGluR5.The activation of SHP-2 may be necessary for group I mGluRs to process the nocicep-tive information.关键词
mGluR1 /5/SHP-2/磷酸酶/脊髓背角/DHPG/NSC-87877Key words
mGluR1 /5/SHP-2/phosphatase/spinal cord dorsal horn/DHPG/NSC-87877分类
医药卫生引用本文复制引用
薛曼,杨云惠,索占伟,杨娴,胡晓东..蛋白酪氨酸磷酸酶 SHP-2在Ⅰ型代谢型谷氨酸受体诱发痛觉超敏中的作用[J].中国药理学通报,2017,33(2):171-174,4.基金项目
国家自然科学基金资助项目 （）
