中国药理学通报2017，Vol.33Issue(2)：175-179,180,6.DOI:10.3969/j.issn.1001-1978.2017.02.007
Lin28B/let-7d 环路调控成纤维细胞功能参与肺纤维化发生
Lin28B/let-7d axis contributes to pulmonary fibrosis by affecting mesenchymal phenotypic properties of lung fibroblasts
摘要
Abstract
Aim To examine the role and uderlying mechanisms of Lin28 /let-7d axis in the proliferation of lung fibrobalsts and fibroblasts-into-myfibroblasts tran-sition,and provide novel strategy for the treatment of idiopathic pulmonary fibrosis (IPF).Methods We induced experimental lung fibrosis in mice by intratra-cheally injection of bleomycin (BLM).Ang Ⅱ and TGF-β1 were used to induce fibrogenesis in cultured MRC-5 cells;qRT-PCR and Western blot were applied to determine the changes of Lin28B,collagen 1 α1 and collagen 3α1 ;MTT assay,Edu satining and immun-ofluoresence were used to examine the cell viability, proliferation and fibroblasts-into-myofibroblasts transi-tion in MRC-5 cells.Results Lin28B was increased in the lung of mice with experimental lung fibrosis and in MRC-5 cells treated with AngⅡ or TGF-β1 .Moreo-ver,Lin28B enhanced collagen deposition via inhibi-ting expression of let-7d,which maybe contribute to the progression of IPF.In addition,further studies showed that Lin28B promoted proliferation and fibro-blasts-into-myofibroblasts in MRC-5 cells.Conclusion Lin28B /let-7d axis contributes to fibrogenesis via promotes fibroblasts-into-myofibroblasts transition, which may provide novel approaches for lung fibrosis treatment.关键词
肺纤维化/Lin28B/let-7d/MRC-5 细胞/肌成纤维细胞/细胞增殖Key words
pulmonary fibrosis/Lin28B/let-7d/MRC-5 cell/myofibroblasts/cell proliferation分类
医药卫生引用本文复制引用
梁海海,李天宇,解岩,单宏丽..Lin28B/let-7d 环路调控成纤维细胞功能参与肺纤维化发生[J].中国药理学通报,2017,33(2):175-179,180,6.基金项目
黑龙江省教育厅科学技术研究资助项目 （）
