中国药理学通报2017，Vol.33Issue(2)：249-254,6.DOI:10.3969/j.issn.1001-1978.2017.02.020
脂肪酸参与糖尿病状态下肝细胞上 CYP1A2功能与表达的上调
Fatty acid participates in up-regulation of diabetes on function and expression of CYP1A2
摘要
Abstract
Aim To investigate the mechanism of dia-betes changing the hepatic CYP1 A2 through in vitro cell culture study.Methods The function of CYP1 A2 in HepG2 and Fa2N-4 cells were evaluated by determi-ning the level of phenacetin metabolism,and the mR-NA expression of CYP1 A2 in cells was detected by real time PCR.HepG2 cells were co-cultured with serum of diabetic rats(type 1 and type 2)and normal rats,then the CYP1 A2 function in cells were evaluated.Then, the HepG2 and Fa2N-4 cells were co-cultured with a series of concentrations of saturated (including palmitic acid and stearic acid)and unsaturated fatty acids(in-cluding oleic acid and linoleic acid)for 48 h,and the function and expression of CYP1 A2 in the cells were compared.Results It was found that the activities of CYP1 A2 were higher in cells incubated with diabetic serum of both type.All high concentration of fatty acids could increase the function and expression of CYP1 A2 in both HepG2 and Fa2N-4 cells.Conclusion It is speculated that the abnormal level of fatty acids under diabetic state might be part of the reasons why diabetes change the hepatic CYP1 A2,which provides the basis for future study.关键词
糖尿病/脂肪酸/HepG2 细胞/Fa2N-4 细胞/CYP1 A2功能/CYP1 A2 mRNA 表达Key words
diabetes/fatty acids/HepG2/Fa2N-4/CYP1 A2 function/CYP1 A2 mRNA expression分类
医药卫生引用本文复制引用
胡楠,蒋艳,韩睿,钱卿,邹素兰..脂肪酸参与糖尿病状态下肝细胞上 CYP1A2功能与表达的上调[J].中国药理学通报,2017,33(2):249-254,6.基金项目
国家自然科学基金青年基金（No 81503136）；常州市卫生人才培养工程 （）
