中国药业2017，Vol.26Issue(1)：10-12,13,4.DOI:10.3969/j.issn.1006-4931.2017.01.003
掺锶聚磷酸钙骨修复材料对成骨细胞血管内皮生长因子蛋白分泌量的影响
Effect of Strontium Doped Calcium Phosphate Bone Repair Material on VEGF Protein Secretion in Osteoblasts
摘要
Abstract
Objective To learn the effect of strontium doped calcium phosphate bone repair material on VEGF protein secretion in os-teoblasts and to provide a new bone repair material for orthopedics clinic. Methods The strontium doped calcium phosphate bone re-pair materials with different strontium content were prepared. The VEGF protein secretion was measured by ELISA method, and the ef-fect of MTT on the proliferation of osteoblasts was investigated. Results The results of ELISA method after 7 d showed that the secre-tion of VEGF in osteoblasts of 1%, 3%, 5%, 8%, 10% strontium doped calcium phosphate were higher than calcium phosphate, and the differences were statistically significant ( t=6. 561, 11. 454, 11. 256, 15. 149, 9. 778, P ﹤ 0. 05 ) . Among them, the content of VEGF was the highest in the 8% calcium containing calcium strontium calcium phosphate. The results of MTT method showed that the A values of 3, 5, 7, 9 d was higher than that of the negative control, and the value of the osteoblasts was higher than that of the negative control, and the differences were statistically significant ( t=3. 960, 5. 029, 5. 229, 4. 808, 2. 584, 2. 954, 2. 657, 2. 798, P ﹤ 0. 05 ) . After culture 5, 7, 9 d, the A value of the osteoblast of the 8% calcium doped calcium phosphate solution were higher than that of calcium phos-phate, and the differences were statistically significant ( t=2. 583, 2. 930, 1. 934, P ﹤ 0. 05 ) . Conclusion As a new bone repair materi-al, strontium doped calcium phosphate can promote the secretion of VEGF, which has a good clinical application prospects.关键词
掺锶聚磷酸钙/骨修复材料/成骨细胞/血管内皮生长因子Key words
strontium doped calcium phosphate/bone repair material/osteoblast/vascular endothelial growth factor分类
医药卫生引用本文复制引用
俞雷钧..掺锶聚磷酸钙骨修复材料对成骨细胞血管内皮生长因子蛋白分泌量的影响[J].中国药业,2017,26(1):10-12,13,4.基金项目
浙江省科学技术厅2014年合作研发和成果转化项目[2014F50005]。 （）
