中国药业2017，Vol.26Issue(1)：28-30,3.DOI:10.3969/j.issn.1006-4931.2017.01.009
高效液相色谱法测定左旋四氢巴马汀自微乳中左旋四氢巴马汀含量
Content Determination of L-Tetrahydropalmatine in L-Tetrahydaopalmatine Self-Microemulsion by HPLC
梁淑贞 1丁少波 1钟李青 1何瑞荣1
作者信息
- 1. 广东省东莞市人民医院药学部，广东 东莞 523000
- 折叠
摘要
Abstract
Objective To establish a content determination method of L-tetrahydropalmatine in L-tetrahydaopalmatine Self-Microemul-sion by HPLC. Methods The column was Phenomenex Desc Gemini-NX 5 μ C18 column(250 mm × 4. 6 mm, 5 μm, 110Å);the mobile phase consisted of 0. 1% phosphoric acid solution ( pH adjusted to 6. 32 with triethylamine ) and methanol ( 35:65 );the detection wave-length was set at 280 nm;the injection volume was 10 μL;the flow rate was 1. 0 mL/min and the column temperature was 30 ℃. Results The calibration curve was linear in the range of 0. 1 to 1. 0 g/L ( r=0. 999 9 ); RSD of accuracy was 0. 38%, and the RSD of stability was 0. 96%. The content of L-tetrahydropalmatine was 91. 81% and the RSD was 0. 58% in the repeatability test. The re-covery rate of L-tetrahydropalmatine was 99. 97% and the RSD was 0. 60%( n=9 ) in the recovery test. Conclusion The method is simple, sensitive, reproducible, accurate and reliable, which can be used as a quality control method for L-tetrahydropalmatine self-mi-croemulsion.关键词
高效液相色谱法/左旋四氢巴马汀自微乳/含量测定Key words
HPLC/L-tetrahydropalmatine Self-Microemulsion/content determination分类
医药卫生引用本文复制引用
梁淑贞,丁少波,钟李青,何瑞荣..高效液相色谱法测定左旋四氢巴马汀自微乳中左旋四氢巴马汀含量[J].中国药业,2017,26(1):28-30,3.
