中国药业2017，Vol.26Issue(2)：16-18,19,4.DOI:10.3969/j.issn.1006-4931.2017.02.005
口虾蛄总生物碱对人肝癌HepG2细胞抗肿瘤作用实验研究
Experimental Studies on the Anti-Tumor Function of the Total Alkaloids of Squilla Oratoria against the Human Hepatoma HepG2 Cell
摘要
Abstract
Objective To extract and analyze the anti-tumor function of the total alkaloids of Squilla oratoria against the human hep-atoma HepG2 cell. Methods The total alkaloids of Squilla oratoria were extracted by acid extraction and alkaline deposition,then isolated by ethyl acetate;CCK-8 assay,plate clone formation assay,flow cytometry,were performed to measure the effect of the total alkaloids of Squilla oratoria on the human hepatoma HepG2 cell. Results The IC50 of cell viability on HepG2 cells after treated with S for 24 h,48 h,and 72 h was 746. 72 μg/mL,47. 58 μg/mL or 26. 32 μg/mL,respectively. After treated with S (0 μg/mL, 10 μg/mL,50 μg/mL and 100 μg/mL)for two weeks,the cloning efficiency of HepG2 cells was 56. 1%,35. 7%,14. 1% or 0,re-spectively;After treated with S for 48 h ,the human hepatoma HepG 2 cells in S stage was increased from 26. 14% to 40. 80%. Conclusion The total alkaloids of Squilla oratoria has an inhibitory effect on the cell growth in HepG2 cells through the Pathways of cell cycle arrest.关键词
口虾蛄/总生物碱/人肝癌HepG2细胞/CCK-8/平板克隆形成试验/细胞周期Key words
squilla oratoria/total alkaloids/human hepatoma HepG2 cell/cell counting kit-8 assay/plate clone formation assay/cell cycle分类
医药卫生引用本文复制引用
王槐高,孔霞,郭向华,顾帝水,李彬彬,周艳芳..口虾蛄总生物碱对人肝癌HepG2细胞抗肿瘤作用实验研究[J].中国药业,2017,26(2):16-18,19,4.基金项目
广东建设中医药强省科研项目[20141155]；广东医学院面上基金项目[2xk1310]。 （）
