中国中医药信息杂志2017，Vol.24Issue(3)：43-47,5.DOI:10.3969/j.issn.1005-5304.2017.03.011
异常黏液质成熟剂对APP/PS1转基因阿尔茨海默病小鼠学习记忆和脑组织RAGE、LRP1蛋白表达的影响
Effects of Abnormal Phlegmatic Munziq on Ability of Learning and Memory and Protein Expressions of Brain Tissue RAGE and LRP1 of APP/PS1 Transgenic Mice Model of AD
摘要
Abstract
Objective To investigate the effects of Abnormal Phlegmatic Munziq on ability of learning and memory, and protein expressions of brain tissue RAGE and LRP1 of APP/PS1 transgenetic mice model of AD;To discuss its mechanism of action. Methods Three-month-old APP/PS1 transgenic mice were randomly divided into 5 groups: model control group, positive control group, Abnormal Phlegmatic Munziq high-, medium-, and low-dose groups, 18 mice in each group. Another 18 three-month-old C57BL/6J mice were chosen as normal control group. All administration groups received relevant medicine for successive 6 months. Then the changes in ability of learning and memory of mice were detected by Step-down test; protein expressions of LRP1 and RAGE were detected by immunohistochemistry and Western blot. Results Compared with the normal control group, the reaction time of learning grades and the mistake times increased, incubation of memory grades decreased and the mistake times increased in the model control group (P<0.01);Compared with the model control group, the reaction time of learning grades and the mistake times decreased, incubation of memory grades increased and the mistake times decreased in all administration groups (P<0.05, P<0.01). Immunohistochemistry and Western blot results showed that compared with normal control group, the LRP1 expression decreased and RAGE increased in the model control group (P<0.05);Compared with the model control group, the LRP1 expression decreased and RAGE increased in Abnormal Phlegmatic Munziq high-, medium-, and low-dose groups (P<0.05,P<0.01). Conclusion Abnormal Phlegmatic Munziq can improve ability of spatial learning and memory in APP/PS1 mice and regulate the expressions of RAGE and LRP1.关键词
异常黏液质成熟剂/APP/PS1转基因小鼠/空间学习记忆/海马CA1区/RAGE/LRP1Key words
Abnormal Phlegmatic Munziq/APP/PS1 transgenic mice/spatial learning and memory/hippocampal CA1/RAGE/LRP1分类
医药卫生引用本文复制引用
塔依尔•吐尔松,阿地兰•沙拉木,兰希,孙龙飞,努尔买买提•艾买提..异常黏液质成熟剂对APP/PS1转基因阿尔茨海默病小鼠学习记忆和脑组织RAGE、LRP1蛋白表达的影响[J].中国中医药信息杂志,2017,24(3):43-47,5.基金项目
教育部新世纪优秀人才计划 （）
