中山大学学报（医学科学版）2017，Vol.38Issue(1)：8-14,7.
前扣带回内肿瘤坏死因子α过表达在神经病理性疼痛中的作用
Role of TNF-αin Anterior Cingulate Cortex in Neuropathic Pain Induced by Sciatic Nerve Injury
摘要
Abstract
Objective]To discuss the effect of sciatic nerve injury on the expressions of tumor necrosis factor-alpha(TNF-α), interleukin-1β(IL-1β)and interleukin-10(IL-10)in anterior cingulate cortex(ACC),and further to explain their roles resided in the development of neuropathic pain.[Method]With use of the methods of behavioral test,western blot and immunohistochemistry, we examine the effects of spared sciatic nerve injury(SNI)on the expressions of TNF-α,IL-1β,and IL-10 in ACC,and observe the effect of the neutralizing antibody of TNF-α,IL-1β on the rat mechanical allodynia.[Result]In present experiment ,SNI increased the protein levels of TNF-α,IL-10,but not IL-1β in ACC. Increased TNF-α-IR and IL-10-IR in ACC is located in neurons ,but not astrocytes and microglia at 7 d following L5-VRT. Pre-treatment with anti-TNFα antibody but not anti-IL-1βantibody into ACC significantly increased the rat paw withdrawal threshold to von Frey hairs.[Conclusion]These data suggested that the increased TNF-αin ACC neurons might be responsible for the development of neuropathic pain.关键词
肿瘤坏死因子-α/前扣带回/神经病理性疼痛Key words
tumor necrosis factor-alpha/anterior cingulate cortex/neuropathic pain分类
医药卫生引用本文复制引用
王少坤,陈少霞,姚沛汶,孙梓乘,那晓东,臧颖..前扣带回内肿瘤坏死因子α过表达在神经病理性疼痛中的作用[J].中山大学学报（医学科学版）,2017,38(1):8-14,7.基金项目
国家自然科学基金（81371228）；广东省科技计划项目（2012B031800254）；广东省自然科学基金（S2013010016647）；中央高校基本科研业务费专项资金 （）
