中山大学学报（医学科学版）2017，Vol.38Issue(1)：56-62,7.
O2·-和H2O2表达上调对2型糖尿病患者血小板异常活化的影响
Effect of Elevated O2·-and H2O2 Expression on Abnormal Activation of Platelets in Patients with Type 2 Diabetes Mellitus
摘要
Abstract
[Objective]To investigate the molecular mechanism of abnormal platelet activation induced by platelet O2 ·- and H2O2 levels in type 2 diabetes mellitus.[Methods]The platelet parameters in patients with type 2 diabetic patients and normal controls were measured;Immunofluorescence technique was used to observe the platelet morphology changing;Flow cytometry was used to detect platelet intracellular O2 ·- and H2O2 content in two groups,then with platelets in normal controls treated with NADH/PMS system and H2O2 respectively,platelet activation positive percentage was observed. Standard Western blot analysis protocols were used to detect expression difference of Catalase and type 2 super-oxide dismutase(Mn-SOD)in platelets.[Results]The MPV in the group of type 2 diabetic patients was significantly higher than in the normal control group(P < 0.001),but there was no statisticdifference in PLT,PDW,PCT between two groups. Immunofluorescence results showed that morphology of platelets in type 2 diabetic patients changed contrast to normal group. Through flowcytometry detection,the content of mitochondrial O2·-and H2O2 of platelet in type 2 diabetic patients were obviously higher than in normal group(P<0.05),whereas no significant difference in cytoplasmic O2·-. We adopted NADH/PMS system and H2O2 to treat platelets of normal group,heightened activated positive percentage were observed which described O2 ·- and H2O2 can significantly promote platelet activation(P<0.01). Western blot results showed that expression of Catalase in platelet of type 2diabetes patients decreased,while the expression and activity of Mn-SOD had no difference.[Conclusion]It is diabetic platelet Catalase expression decreased that may lead to Diabetic platelet mitochondrial O 2 ·- and H2O2 level increased ,thus regulating aberrant activation of diabetic platelet.关键词
2型糖尿病/血小板活化/平均血小板体积/O2·-/H2O2Key words
type 2 diabetes patients/platelet activation/MPV/O2·-/H2O2分类
医药卫生引用本文复制引用
陈菲,罗创华,卢汉平,周倜,杨中汉,杨霞,高国全,齐炜炜..O2·-和H2O2表达上调对2型糖尿病患者血小板异常活化的影响[J].中山大学学报（医学科学版）,2017,38(1):56-62,7.基金项目
国家自然科学基金（81471033，81600641）；广东省自然科学基金（2014A030313073，2015A030311043，，2016A030311035）；广州市科技计划产学研协同创新重大专项（201508020033）；中山大学高校基本科研业务费青年教师科研资助计划项目（16ykpy24）；广东省高等学校高层次人才项目；国家大学生创新团队项目 （）
