中山大学学报（医学科学版）2017，Vol.38Issue(1)：106-112,7.
LOC389023在难治性颞叶癫痫中分子海绵机制的预测
Forecasting of Molecular Sponge Mechanism Mediated by LOC389023 in Patients with Intractable Temporal Lobe Epilepsy
摘要
Abstract
[Objective]To forecast the sponge mechanism mediated by LOC389023 in patients with intractable temporal lobe epilepsy(TLE),through investigating the expression of microRNA interacted with dipeptidyl peptidase 10(DPP10)and LOC389023.[Methods]The expression of DPP10 and Kv4.3 were detected in 15 temporal neocortex from patients with brain trauma (control group)and in 26 temporal neocortex from patients with intractable TLE(epilepsy group)by western blot(WB)and immunohisto⁃chemical(IHC)staining. The location of DPP10 and voltage dependent potassium channel 4.3(Kv4.3)was detected by immunofluo⁃rescent(IF)staining. The interaction between DPP10 and Kv4.3 was testified by co-immunoprecipitation(Co-IP). The expression of microRNA obtained by softwares(miRanda,Pita,TargetScan and miRDB)was detected by qPCR.[Results]IHC and WB showed an increased expression of DPP10(P<0.05)and a decreased expression of Kv4.3(P<0.05)in the epilepsy group. IF showed that the DPP10 and the Kv4.3 co-expressed in the membrane and the cytoplasm of neurons. Co-IP showed obvious interaction between the DPP10 and the Kv4.3.Five microRNA(miR-32-5p,miR-140-5p,miR-367-3p,miR-25-3p,miR-4325)were obtained by soft⁃wares. No significant differences in the expression of miR-32-5p and miR-4325 were found between epilepsy group and control group by qPCR(P>0.05). But decreased expression of LOC389023 and miR-140-5p and increased expression of miR-25-3p and miR-367-3p were found in epilepsygroup compared to control group (P < 0.05).[Conclusion]miR-25-3p and miR-367-3p may be regulated by LOC389023 through the sponge mechanism followed by altered expression of DPP10 in intractable temporal lobe epilepsy.关键词
难治性颞叶癫痫/分子海绵机制/LOC389023/DPP10/Kv4.3Key words
intractable temporal lobe epilepsy/molecularsponge mechanism/LOC389023/DPP10/Kv4.3分类
医药卫生引用本文复制引用
温跃桃,姜维,吴昆仑,石全红..LOC389023在难治性颞叶癫痫中分子海绵机制的预测[J].中山大学学报（医学科学版）,2017,38(1):106-112,7.基金项目
重庆市卫生局重点资助项目 （）
