作物学报2017，Vol.43Issue(2)：179-189,11.DOI:10.3724/SP.J.1006.2017.00179
甘蓝型油菜盐胁迫下幼苗鲜重和干重QTL定位及候选基因分析
QTL Mapping for Seedling Dry Weight and Fresh Weight under Salt Stress and Candidate Genes Analysis inBrassica napusL.
摘要
Abstract
Salt stress is one of the main abiotic stresses affecting crop yield and it would be very important by using the salt tole-rance related markers in rapeseed breeding to improve the oilseed production. In this research, the Brassica napus L. high genera-tion recombinant inbred lines (RIL) population derived from the cross of GH06 and P174 via single seed descent propagation was used for QTL mapping and candidate gene analysis. The fresh and dry weight of leaf and root were measured at 25 days after the seedlings were grown in Hoagland solution with 16 g L–1NaCl. Composite interval mapping (CIM) was used to identify the re-lated QTLs according to the high density SNP genetic map, and the candidate gene expression in the extreme lines tested by qRT-PCR. A total of 19 QTLs were identified in the control and salt stress treatment, and six QTLs were mapped on chromosomes A02, A04, and C03 under salt stress, with contribution rate ranged from 7.16% to 16.15%. Eight genes were identified according to the BLAST of genes in the QTL confidence intervals and the salt stress related genes inArabidopsis. The expression of four candidate genes in the extreme lines showed thatBnaA02g14680D andBnaA02g14490D under salt stress treatment for 48 or 72 hours had higher expression than the control, which indicates that the expressions are induced by salt stress. The relative expres-sions of gene BnaC03g64030Din sensitive extreme lines were higher than those in tolerant extreme lines. There were no changed in expression for gene BnaC03g62830D in sensitive extreme lines but increased expression at 48 hours and reduced expression at 72 hours after salt treatment in tolerant extreme lines, showing the enhance of plant salt tolerance possibly. Our research laid a foundation for the function research of salt tolerant gene in rapeseed and the breeding of salt tolerant rapeseed.关键词
甘蓝型油菜/盐胁迫/数量性状位点/单核苷酸多态性/候选基因Key words
Brassica napusL/Salt stress/QTL/SNP/Candidate gene引用本文复制引用
侯林涛,王腾岳,荐红举,王嘉,李加纳,刘列钊..甘蓝型油菜盐胁迫下幼苗鲜重和干重QTL定位及候选基因分析[J].作物学报,2017,43(2):179-189,11.基金项目
本研究由国家自然科学基金项目(31371655)资助。The study was supported by the National Natural Science Foundation of China (31371655) （31371655）
