作物学报2017，Vol.43Issue(2)：190-200,11.DOI:10.3724/SP.J.1006.2017.00190
高粱Na+转运蛋白基因SbSKC1的克隆及其在烟草中的抗盐功能鉴定
Cloning ofSbSKC1Encoding Na+ Transporter Protein from Sorghum and Its Function of Salt-Resistance in Tobacco
摘要
Abstract
The Na+ transporter proteinplays an important role in response to abiotic stresses in plant. A Na+ transporter gene, SbSKC1(XM_002457691.1), was cloned fromSorghum bicolor in this study.Its full-length open reading frame comprises 1497 nucleotides and encodes 498 amino acids. Multiple sequence alignment and phylogenetic analysis indicated thatSbSKC1shares a high similarity withLOC100382359 (NP_001162576.1) fromZea mays.SbSKC1was incorporated in the vector pSH-SbSKC1 and transferred into tobacco (Nicotiana tabacumcv. Xanthin) viaAgrobacterium-mediated transformation. The transgenic tobacco plants were validated by PCR assay and subject to 300 mmol L–1 NaCl stress. The results showed higher survival rate and signifi-cantly longer roots in the transgenic plants than in the wild type. Besides, the transgenic plants maintained higher level K+/Na+ ratio. Under salt stress, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were significantly higher in the transgenic tobacco than in the wild type. However, the content of hydrogen peroxide (H2O2) was 37.7% lower in the transgenic tobacco than in the wild type. These results suggest that overexpression ofSbSKC1 may improve the salt resistant of tobacco.关键词
烟草/SbSKC1基因/抗盐性/抗氧化性Key words
Tobacco/SbSKC1gene/Salt resistance/Oxidation resistance引用本文复制引用
姚新转,刘洋,赵德刚..高粱Na+转运蛋白基因SbSKC1的克隆及其在烟草中的抗盐功能鉴定[J].作物学报,2017,43(2):190-200,11.基金项目
本研究由国家自然科学基金项目(31160149),贵州省科技厅农业攻关项目(黔科合 NZ 字[2012]3009号)和国家转基因生物新品种培育重大专项(2014ZX08010-003)资助。This study was supported by the National Natural Science Foundation of China (31160149), the Agriculture Research Project from Guizhou Department of Science and Technology (NZ[2012]3009), and the National Major Project for Developing New GM Crops (2014ZX08010-003) （31160149）
