作物学报2017，Vol.43Issue(2)：201-209,9.DOI:10.3724/SP.J.1006.2017.00201
小麦TaVIP1家族基因克隆、分子特性及功能分析
Cloning, Molecular Characterization, and Functional Analysis of Wheat TaVIP1 Genes
摘要
Abstract
Plant VIP1 (VirE2 interacting protein 1) is involved in the transportation of T-DNA in plant cells afterAgrobacterium infection, affecting plant transformation efficiency. However, the function ofTaVIP1 is unknown. Using in silico technique, a family ofTaVIP1 genes were successfully cloned from common wheat in this study. The gene family encompasses four exons and shares high similarity among its members while only shares 50.7%–51.4% similarity to the AtVIP1 inArabidopsis. Southern blot-ting assay showed that theTaVIP1 had three allelic copies in wheat genome. The three copies ofTaVIP1 were further assigned to wheat chromosomes 4AL, 4BS, and 4DS according to Blastn in the IWGSC (International Wheat Genome Sequencing Consor-tium) database, and experimentally confirmed by PCR using specific primers to eachTaVIP1 allele based on their DNA sequences and Langdon-Chinese Spring disomic substitution lines. Subcellular localization analysis showed that TaVIP1 proteins were lo-cated in cell membrane, cytoplasm, and nucleus. Over-expression of TaVIP1 in tobacco obviously reducedAgrobacte-rium-mediated transformation efficiency. The amino acid sequence encoded byTaVIP1 only had low similarity to the correspond-ing amino acid sequence encoded byAtVIP1 inArabidopsis orNtVIP1 in tobacco. This might be a reason of the low transforma-tion efficiency of wheat mediated byAgrobacterium.关键词
小麦/TaVIP1/烟草/农杆菌转化Key words
Wheat/TaVIP1/Tobacco/Agrobacterium-mediated transformation引用本文复制引用
赵佩,腾丽杰,王轲,杜丽璞,任贤,佘茂云,叶兴国..小麦TaVIP1家族基因克隆、分子特性及功能分析[J].作物学报,2017,43(2):201-209,9.基金项目
本研究由国家自然科学基金项目(31401380,31401376)资助。This study was supported by the National Natural Science Foundation of China (31401380,31401376) （31401380,31401376）
