作物学报2017，Vol.43Issue(2)：210-217,8.DOI:10.3724/SP.J.1006.2017.00210
基于数字基因表达谱分析的茶树花不育基因挖掘
Exploring Sterility Gene from Tea Plant Flower Based on Digital Gene Expres-sion Profiling
摘要
Abstract
Tea tree (Camellia sinensis) blossoms every year and a lot of nutrients are consumed by itself during blooming and fruiting, resulting in decreased yield and quality of fresh tea leaf. In the present study, the flowers of Fuding Dabaicha (male par-ent), Foxiang 2 (female parent) and their hybrid (sterile flowers) were used to construct cDNA libraries and the gene expression profiles were analyzed by Solexa sequencing technology. A total of 1219 candidate sterility genes were identified according to their differential expression between the male/female parent and the hybrid but identical between the two parents. These sterility gene were subject to GO analysis, and metabolic process, hydrolase activity and catalytic activity were enriched. KEGG pathway analysis showed that the sterility genes were involved in biosynthesis of amino acids and carbohydrate, second any metabolism, plant hormone signal transduction, and energy metabolism. In the function group of plant hormone signal transduction, 16 anno-tated genes were involved in the auxin signal transduction pathway, including five up-regulatedAFR genes in the hybrid and 11 down-regulated genes. Thus, we speculate that auxin signal transduction might regulate the flower sterility in tea tree. Five steril- ity genes were selected randomly for qRT-PCR assay and the result was consistent with that of Solexa sequencing. The candidate sterility genes found in this study are worthy of sterile mechanism research in tea plant and sterility gene screening.关键词
茶树花/基因表达谱/不育基因/差异表达基因Key words
Tea plant flowers/Gene expression profiles/Sterility gene/Differentially expressed genes引用本文复制引用
陈林波,夏丽飞,田易萍,李梅,宋维希,梁名志,江昌俊..基于数字基因表达谱分析的茶树花不育基因挖掘[J].作物学报,2017,43(2):210-217,8.基金项目
本研究由国家自然科学基金项目(31560220,31460216),云南省人才培养计划项目(2015HB105),茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20150105)资助。This study was supported by the National Natural Science Foundation of China (31560220,31460216), the Personnel Training Program of Yunnan Provence (2015HB105), and the Open Fund of State Key Laboratory of Tea Plant Biology and Utilization (SKLTOF20150105) （31560220,31460216）
