作物学报2017，Vol.43Issue(2)：218-225,8.DOI:10.3724/SP.J.1006.2017.00218
花生AhHDA1互作蛋白AhGLK的筛选及特性分析
Screening of AhHDA1 Interacting-Protein AhGLK and Characterization in Peanut (Arachis hypogaeaL.)
摘要
Abstract
With AhHDA1 as bait, the peanut (Arachis hypogaeaL.) cDNA library were screened and the characteristics of the interacting-protein was analysed. The yeast two-hybrid experiment indicated thatArachis hypogaea Golden 2-like (AhGLK) in-teracts with AhHDA1, and interaction between AhHDA1 and AhGLK was further confirmed by bimolecular fluorescence com-plementation (BiFC) assay. There has a MYB conserved domain in AhGLK, and the domain shows high homology with GsGLK (Glyine soja) and AtGLK (Arabidopsis thaliana) though bioinformatics software analysis. AhGLK was found located in nucleus and was testified as a transcriptional activation factor. Tissue specific analysis showed thatAhGLK accumulated in leaves mainly, and the expression level was down-regulated under 30% PEG treatment. Above all, the results lay the foundation for further study onAhGLK, which might be involved in drought stress resistance of peanut.关键词
花生/AhHDA1/蛋白互作/AhGLK/转录活性Key words
Peanut/AhHDA1/Interacting protein/AhGLK/Transcriptional activity引用本文复制引用
李媚娟,苏良辰,刘帅,李晓云,李玲..花生AhHDA1互作蛋白AhGLK的筛选及特性分析[J].作物学报,2017,43(2):218-225,8.基金项目
本研究由国家自然科学基金项目(31671600)资助。This study was supported by the National Natural Science Foundation of China (31671600) （31671600）
