作物学报2017，Vol.43Issue(2)：307-312,6.DOI:10.3724/SP.J.1006.2017.00307
与小麦抗白粉病基因Pm48紧密连锁分子标记的开发
Development of Markers Closely Linked with Wheat Powdery Mildew Resis-tance GenePm48
摘要
Abstract
Pm48 is a novel powdery mildew resistance gene identified previously in our laboratory. This study aimed at develop-ing close molecular markers for fine mapping of the gene. The ddRAD-sequencing assay revealed 81 SNPs associated with the target gene, in which one converted into the STS markerXmp931 and three converted into the CAPS markersXmp928,Xmp930, andXmp936. We also developed 71 genomic SSR markers according to the genome sequence ofAegilops tauschii. And mapped two of them,Xmp1089 and Xmp1112. Using the 115 F2:3 families derived from the cross of Ningnuomai 1´ Tabasco, the target gene was found to be co-segregated withXmp928 and distal toXmp1112 with the genetic distance of 3.1 cM towards centromere. In the 671 homozygous susceptible families,Xmp928 also showed co-segregated with the target gene. We also physically mapped Pm48 to the bin of 5DS 0.63–0.67 by using three Chinese Spring 5DS deletion lines.关键词
小麦/抗白粉病基因/分子标记/混池ddRAD测序Key words
Wheat/Powdery mildew resistance gene/Molecular markers/Bulked ddRAD-seq引用本文复制引用
付必胜,刘颖,张巧凤,吴小有,高海东,蔡士宾,戴廷波,吴纪中..与小麦抗白粉病基因Pm48紧密连锁分子标记的开发[J].作物学报,2017,43(2):307-312,6.基金项目
本研究由国家科技支撑计划项目(2013BAD01B02-12),国家现代农业产业技术体系建设专项(CARS-3-1-17),江苏省农业科技自主创新资金项目(CX(14)5006)和江苏省自然科学基金(BK2012783)资助。This study was supported by the National Key Technology R&D Program of China (2013BAD01B02-12), the China Agriculture Research System (CARS-3-1-17), Jiangsu Provincial Foundation of Agricultural Scientific Innovation [CX (14)5006], and the Natural Science Foundation of Jiangsu Province (BK2012783) （2013BAD01B02-12）
