安徽农业科学2017，Vol.45Issue(3)：1-3,18,4.
2种野外采集白腐真菌分离及其产漆酶液体培养基优化
Isolation of Two Species of Field Acquisition White Rot Fungi and Optimization of Liquid Culture Medium of Their Produce Laccase
摘要
Abstract
[Objective] The aim was to optimize the liquid culture medium of two species of field acquisition white rot fungi.[ Method] By isola-ting and purifying two species of white rot fungi,Stereum ostrea and Pycnoporus cinnabarinus,white rot fungus hyphae were collected, and the liq-uid culture medium of laccase was screened.ABTS method was applied to measure the activity of laccase.Effects of carbon sources,nitrogen sources,metal cations,pH,temperature and rotational speed on laccase produced by white rot fungi were studied.[Result] The optimum medium of Stereum ostrea was glucose 20.00 g,potassium nitrate 5.00 g,NaH2 PO45.00 g,CuSO41.00 g,the initial pH 6,temperature 25 ℃, rotational speed 170 r/min,and the laccase activity reached the maximum on the 11th day the maximum value reached 1.557 U/mL which was 2.7 times of the laccase activity of PDA liquid culture.The optimum medium of Pycnoporus cinnabarinus was maltose 25.00 g,yeast extract 5.00 g, NaH2 PO41.00 g,KCl 2.00 g,the initial pH 7,temperature 30 ℃,rotational speed 200 r/min,and the laccase activity reached the maximum in the 9 d,the maximum value 1.478 U/mL which was 2.3 times of the laccase activity of PDA liquid culture.[Conclusion] The opitimal liquid cul-ture medium of two species of field acquisition white rot fungi is obtained so as to provide reference for further study of laccase.关键词
白腐真菌/漆酶/培养基/筛选Key words
White rot fungus/Laccase/Culture medium/Screen分类
农业科技引用本文复制引用
李环明,杨洪升,薛春梅..2种野外采集白腐真菌分离及其产漆酶液体培养基优化[J].安徽农业科学,2017,45(3):1-3,18,4.基金项目
黑龙江省教育厅科学技术研究面上项目( 12531696 ) （ 12531696 ）
佳木斯大学研究生科技创新项目(YM2016_002). （YM2016_002）
