安徽农业科学2017，Vol.45Issue(3)：10-12,30,4.
龙牙百合鳞茎总黄酮提取工艺及抗氧化性研究
Study on the Extraction Technology and Antioxidant Property of Total Flavonoids from Longya lilium Bulb
摘要
Abstract
[Objective] To explore the optimum extraction technology of total flavonoids from Longya lilium bulb and study their antioxidant ac-tivity.[Method] Taking L.lilium bulb as research object,total flavonoids were extracted from L.lilium bulb by using optimized ultrasonic method. Based on single factor experiment,the effects of ethanol concentration,material-liquid ratio,extraction time and extraction temperature on the ex-traction quantity of total flavonoids from L.lilium bulb were studied and the optimum extraction technology was confirmed by using orthogonal ex-periment.And their antioxidant activities of total flavonoids in vitro were studied.[Result] The optimum extraction conditions of total flavonoids from L.lilium bulb were as follows:80% ethanol,solid-liquid ratio of 1:10 (g :mL),extraction temperature of 30 ℃,extraction time of 20 min. Under the optimum conditions,the extraction quantity of total flavonoids was (32.236 ±0.513) mg/g.The results of in vitro antioxidant test showed that the scavenging effects of total flavonoids extract from L.lilium bulb to DPPH? was better than that of vitamin C solution at the same concentration,and there was significant difference(P<0.05).[Conclusion] The research can provide theoretical basis for the comprehensive ex-ploration and utilization of L.lilium.关键词
龙牙百合鳞茎/总黄酮/超声波法/抗氧化活性Key words
Longya lilium bulb/Total flavonoids/Ultrasonic method/Antioxidant activity分类
农业科技引用本文复制引用
薛梅,周卫平..龙牙百合鳞茎总黄酮提取工艺及抗氧化性研究[J].安徽农业科学,2017,45(3):10-12,30,4.基金项目
重庆市教委科学技术研究项目(KJ1401129). （KJ1401129）
