高校化学工程学报2017，Vol.31Issue(1)：126-132,7.DOI:10.3969/j.issn.1003-9015.2017.01.018
循环回流发酵与膜分离、泡沫分离耦合工艺生产多粘菌素E
Production of Polymyxin E via a Circulating Reflux Fermentation, Membrane Separation and Foam Separation Coupled Technique
摘要
Abstract
A fermentation, foam fractionation and membrane separation coupled technique was proposed to increase the titer of polymyxin E production, which used a peristaltic pump to build a circulating reflux process. The advantages of this technique include extended bacteria growth period, reduced product inhibition, less cell entrainment in foam and sufficient nutrition. Moreover, the effects of coupling starting time, interval time, flow rate and single separation time on thetiter of polymyxin E were investigated. The results show that u nder the optimal conditions of coupling starting time 23.0 h, interval time 1.0 h, flow rate 20 mL?min-1 and single separation time 45 min, the polymyxin E titer is 28634 U?mL-1, which increases by 21% when compared with the control group (23597 U?mL-1). Overall, this study improves the titer of polymyxin E and is useful for the separation of other surface-active substances, especially proteins.关键词
发酵/发酵与泡沫分离耦合/循环回流/多粘菌素EKey words
fermentation/fermentation coupling with foam separation/circulating reflux/polymyxin E分类
化学化工引用本文复制引用
徐丹丹,郑辉杰,高迎迎,吴兆亮..循环回流发酵与膜分离、泡沫分离耦合工艺生产多粘菌素E[J].高校化学工程学报,2017,31(1):126-132,7.基金项目
河北省高等学校自然科学研究重点项目(ZD2016072). （ZD2016072）
