解放军医药杂志2017，Vol.29Issue(2)：43-47,5.DOI:10.3969/j.issn.2095-140X.2017.02.011
布拉氏酵母菌对大鼠肠道菌群及血清炎性因子的干预研究
A Study on Saccharomyces Boulardii in Intervention of Intestinal Flora and Inflammatory Factors of Rats
摘要
Abstract
Objective To observe influence of high-fat diet on intestinal flora and inflammatory factors of rats, and to investigate effect of Saccharomyces Boulardii powders in regulation of intestinal flora. Methods A total of 36 rats were randomly divided into group A, B and C ( n=12 for each group) . Group A was given normal diet. Group B was given high-fat diet. Group C was given high-fat diet and Saccharomyces boulardii powder. Lipopolysaccharide (LPS), interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in serum and numbers of bacillus bifidus, lactobacilli, bacte-roides, escherichia coli and enterococcus in feces at the beginning and at the end of 10 th weeks of experiment were detec-ted. Results There were no significant differences in LPS, TNF-αand IL-6 levels at the beginning experiment among 3 groups (P>0. 05). At the end of 10th weeks of experiment, LPS and TNF-αlevels in group B and C were significantly increased (P<0. 05, P<0. 01), while the levels showed no changes in group A (P>0. 05);IL-6 level in group B was significantly increased (P<0. 01), while the levels showed no changes in group A and C compared with those at the be-ginning experiment (P>0. 05). Levels of LPS, TNF-αand IL-6 in group B were significantly higher than those in group A and C (P<0. 01), TNF-αlevel in group C was significantly higher than that in group A at the end of 10th weeks of ex-periment ( P<0. 01 ) . There were no significant differences in numbers of fecal bacillus bifidus, lactobacilli, bacte-roides, escherichia coli and enterococcus at the beginning experiment among 3 groups ( P>0. 05 ) . At the end of 10 th weeks of experiment, numbers of fecal lactobacilli, bacillus bifidus and escherichia coli in group B and C were signifi-cantly higher (P<0. 01), while the numbers showed no changes in group A compared with those at the beginning experi-ment (P>0. 05);the numbers in group B were significantly lower than those in group A and C (P<0. 01), and the numbers in group C were lower than those in group A (P<0. 05). At the end of 10th weeks of experiment, number of fe-cal bacteroides in group B was lower (P<0. 01), while the numbers showed no changes in group A and C compared with those at the beginning experiment (P>0. 05), and the number in group B was significantly lower than those in group A and C (P<0. 01);the number of fecal escherichia coli in group B was significantly higher (P<0. 01), while the num-ber in group A showed no change (P>0. 05), but the number in group C was significantly lower compared with those at the beginning experiment (P<0. 05);the number of fecal escherichia coli in group B was higher than those in group A and C (P<0. 01), and the number in group A was higher than that in group C (P<0. 05). Conclusion High-fat diet may increase release of inflammatory factors and change intestinal flora of rats. Saccharomyces boulardii can stabilize in-testinal flora and reduce release of inflammatory factors.关键词
布拉氏酵母菌/高脂饮食/肠道菌群/炎性细胞因子/大鼠,Sprague-DawleyKey words
Saccharomyces boulardii/High-fat diet/Intestinal flora/Inflammatory cytokines/Rats/Sprague-Dawley分类
医药卫生引用本文复制引用
李超,刘星宇,贺星,王晓辉,闫志辉,弓三东,郑岩,崔立红..布拉氏酵母菌对大鼠肠道菌群及血清炎性因子的干预研究[J].解放军医药杂志,2017,29(2):43-47,5.基金项目
海军后勤科研计划课题(CHJ12J027) （CHJ12J027）
总后勤部卫生部青年培育项目(13QNT073) （13QNT073）
