解放军医药杂志2017，Vol.29Issue(2)：107-110,4.DOI:10.3969/j.issn.2095-140X.2017.02.027
单人份核酸检测在降低输血感染人类免疫缺陷病毒残余风险中的应用
Application of Individual Donor-nucleic Acid Amplification Test in Reducing Residual Risk of Transfusion-trans-missible HIV Infection
摘要
Abstract
Objective To analyze detection capability of individual donor-nucleic acid amplification test ( ID-NAT) for HIV-positive blood samples during window period and to investigate effect of ID-NAT in reducing residual risk of transfusion-transmissible human immunoddficiency virus ( HIV) infection. Methods Blood samples were performed parallel detection by ID-NAT ( Procleix Tigris) and two kinds of enzyme linked immunosorbent assay ( ELISA) reagents from different manufactories, and then the samples with negative by ELISA detection and positive by ID-NAT detection were tested by HIV identification assay. The blood donors with positive HIV identification assay were made tracing tests. Results A total of 196900 blood samples were collected, in which 256 samples were negative by ELISA detection and positive by ID-NAT combined detection, and 2 samples were positive by HIV identification assay. The first HIV-positive blood donor was confirmed positive HIV-1 antibody 29 d after blood donation by western blot confirmatory test, and posi-tive HIV(1+2) antibody and HIV-1 P24 antigen were found by two kinds of ELISA reagents detection. Blood sample of another HIV-positive blood donor showed a elevation trend in virus load 0, 4 and 7 d after blood donation;only one kind of ELISA reagent detection was positive 4 d after blood donation, but both of two kinds of ELISA reagents detection were positive 7 and 14 d after blood donation, and positive HIV-1 antibody was confirmed 30 d after blood donation. Conclu-sion The application of ID-NAT in blood screening can shorten window period of HIV detection and reduce residual risk of transfusion-transmissible HIV infection, and then blood safety can be effectively improved.关键词
输血/血液筛查/单人份核酸检测/窗口期/人类免疫缺陷病毒感染Key words
Blood transfusion/Blood screening/Individual nucleic acid test/Window period/Human immu-noddficiency virus infection/分类
医药卫生引用本文复制引用
李莉华,王素玲,马印图..单人份核酸检测在降低输血感染人类免疫缺陷病毒残余风险中的应用[J].解放军医药杂志,2017,29(2):107-110,4.基金项目
河北省卫计委指令性课题(ZL-20140122) （ZL-20140122）
