中北大学学报（自然科学版）2017，Vol.38Issue(1)：87-92,98,7.DOI:10.3969/j.issn.1673-3193.2017.01.016
基于微流控芯片的细胞排列和迁移的实验研究
Experimental Research of Cell Patterning and Transportation Based on the Microfluidic Chip
摘要
Abstract
Due to cellular heterogeneity,cell patterning and transportation are usually the basic problems that need to be solved before other sophisticated cell electro-manipulations.A microfluidic chip with the parallel electrodes was used to investigate the transportation of human esophageal squamous carcinoma KYSE180 cells;through a microfluidic chip based on the parallel electrodes with different gaps,the transportations of the mixed cells of leukemia NB4 cells and yeast cells were studied;the transportation movement of the two kinds of cells vary along with the changes of the width of electrodes and the gap-width of electrodes was found through COMSOL Multiphysics.The experimental results illustrate that the frequency of the E-field from the signal applied to the electrodes and the conductivity of cell suspen-sion decide cell transportation;the study of transportation experiments of the mixed cells show that the optimal frequency of cell sorting can be acquired easily under the above conditions and establishes the foundation for cell sorting based on complicated chip-structures.关键词
细胞排列/细胞迁移/平行电极/微流控芯片/混合细胞/细胞电操作Key words
cell patterning/cell transportation/parallel electrodesv/microfluidic chip/mixed cells/cell electro-manipulations分类
信息技术与安全科学引用本文复制引用
白国花,谭秋林..基于微流控芯片的细胞排列和迁移的实验研究[J].中北大学学报（自然科学版）,2017,38(1):87-92,98,7.基金项目
山西省青年拔尖支持计划项目 （）
山西省百人计划项目 （）
