山东医药2017，Vol.57Issue(5)：1-3,3.DOI:10.3969/j.issn.1002-266X.2017.05.001
树突状细胞与黑色素瘤细胞体外共培养体系对小鼠黑色素瘤形成的影响
Effect of co-culturing dendritic cells with melanoma cells on formation of melanoma in mice
摘要
Abstract
Objective To investigate the effect of co-culturing dendritic cells (DC)with melanoma cells (B16)on growth of melanoma in mice.Methods We extracted the bone marrow cells.Mouse DC were cultured for 6 days and then were divided into 4 groups:lipopolysaccharide (LPS)group,Poly (IC)group,Melan-A group and the control group. Cells in the LPS group,Poly (IC)group,Melan-A group were incubated with LPS (LPS with final concentration of 100 ng/mL),poly-inosinic-cytidylic acid [Poly(IC)with final concentration of 20 ng/mL]and Melan-A peptide (with final concentration of 5 μmol/L),respectively.The control group was not treated.Flow cytometry was used to study the matura-tion and activation state of DC.B16-F10 (B16)melanoma cells were co-cultured with DC for 2 days and then were used for inoculation of mice.For melanoma model,C57BL/6J mice were inoculated subcutaneously.Some mice were killed at 14 days after inoculation and their spleens were taken to study the structural changes by histochemistry.Tumor size was meas-ured at 28 days after inoculation.Results More mature DC were produced after LPS,Poly(IC)or Melan-A peptide treat-ment as compared with that of the control group (P <0.05).There was no tumor growth after DC +B16 cell inoculation. Compared with the mice inoculated with B16 cells only,the tumor volume was smaller in the mice inoculated with LPS,Po-ly(IC)or Melan-A peptide-activated DC +B16 cells or the mice inoculated with B16 cells one week later followed by an-other inoculation of DC +B16 cells (all P <0.05).Compared with the control group,there was no structural change of the spleen follicles in the mice inoculated with DC +B16,but the number of spleen follicles was increased in the mice inocula-ted with B16 or with LPS,Poly (IC),or Melan-A peptide-activated DC +B16 cells.Conclusion DC co-cultured with B16 can inhibit the growth of melanoma in mice.关键词
黑色素瘤/树突状细胞/免疫治疗/小鼠Key words
melanoma/dendritic cells/immunotherapy/mice分类
医药卫生引用本文复制引用
郑云梅,和晨辰,王世忠,沈万秋,李海东..树突状细胞与黑色素瘤细胞体外共培养体系对小鼠黑色素瘤形成的影响[J].山东医药,2017,57(5):1-3,3.基金项目
国家自然科学基金资助项目(21373151). （21373151）
