山东医药2017，Vol.57Issue(5)：4-7,4.DOI:10.3969/j.issn.1002-266X.2017.05.002
非诺贝特对人BMEC与小鼠脑微血管组织SOD3表达的影响及机制
Effects of fenofibrate on SOD3 expression in human BMECs and mouse brain microvascular tissues
摘要
Abstract
Objective To investigate the regulating effects of fenofibrate,the agonists of peroxisome proliferator-acti-vated receptor α(PPARα),on the superoxide dismutase 3 (SOD3)expression in human brain microvascular endothelial cells (BMECs)and mouse brain microvascular tissues as well as the possible mechanism.Methods The cultured BMECs were randomly divided into two groups,which were seperately treated with 10 μg/ml of fenofibrate and the same volume of DMSO for 12 h.Fifteen mice were divided into two groups,which were treated with 30 mg/kg fenofibrate and 10 mL/kg of 10% CMC by gavage for 7 days.The tRNA was extracted and the brain microvessel was isolated.The mRNA ex-pression levels of PPARαand SOD3 were detected by RT-PCR.We constructed a luciferase reporter plasmid pGL4 mSOD3 containing SOD3 promoter.The luciferase reporter plasmid pGL4 mu mSOD3 mutated from PPRE sequence were obtained by using the mutagenesis kit.The BMECs were transfected with pGL4 mSOD3 and pGL4 mu mSOD3 for 24 h.The cells were treated with fenofibrate at a final concentration of 10 μg/mL for 12 h in order to measure the luciferase activity.Re-sults The mRNA expression of PPARαand SOD3 was increased in the BMECs and brain microvessel after the treatment of fenofibrate (all P <0.05).The luciferase activity of pGL4 mSOD3 in BMECs treated with fenofibrate was enhanced after transfection of pGL4 mSOD3 for 24 h (P <0.05).The luciferase activity of BMECs treated with fenofibrate did not signifi-cantly change after transfection of pGL4 mu mSOD3 for 24 h (P >0.05).Conclusion The SOD3 expression in the human BMECs and mouse brain microvascular tissues is regulated by fenofibrate through activating PPARα.关键词
非诺贝特/细胞外铜/锌超氧化物歧化酶/脑微血管/脑微血管内皮细胞/小鼠Key words
fenofibrate/extracellular Cu/Zn superoxide dismutase/brain microvascular/brain microvascular endo-thelial cells/mice分类
医药卫生引用本文复制引用
杜小珊,杨锡彤,徐弘扬,程建杰,王光明..非诺贝特对人BMEC与小鼠脑微血管组织SOD3表达的影响及机制[J].山东医药,2017,57(5):4-7,4.基金项目
国家自然科学基金资助项目(81360206) （81360206）
云南省中青年学术技术带头人后备人才基金项目(2014HB025). （2014HB025）
