山东医药2017，Vol.57Issue(5)：12-14,3.DOI:10.3969/j.issn.1002-266X.2017.05.004
eotaxin-3基因+2497、+77位点SNP单核苷酸多态性与支气管哮喘易感性及类型的关系
Relationship of eotaxin-3 +2497 and +77 SNP with types and susceptibility of bronchial asthma
摘要
Abstract
Objective To investigate the relationships of eotaxin-3 gene single nucleotide polymorphism (SNP)with susceptibility to asthma,types and the levels of eotaxin-3.Methods We recruited 196 healthy control subjects (control group)and 196 patients with asthma (asthma group),including 140 cease of eosinophilic asthma (EA)and 56 cases of non-eosinophilic asthma (NEA).The polymorphism,allele frequency and genotype frequency of eotaxin-3 +77C >T,+2497T >G were detected by PCR-RFLP.ELISA was used to detect the level of eotaxin-3 in plasma.Results The geno-type frequency of eotaxin-3 +2497 TG and allele frequency of eotaxin-3 +2497G in the asthma group were significantly higher than those of the control group (all P <0.05).However,the allele frequency of eotaxin-3 +77T and genotype fre-quency of eotaxin-3 +77 showed no difference between the asthma and control groups (all P >0.562).The frequency of eotaxin-3 +2497 TG genotype in NEA patients was significantly higher than that in EA patients (P <0.05).The level of eotaxin-3 in TG genotype asthmatic patients was significantly lower than that in TT genotype (P <0.05).Conclusions Eotaxin-3 +2497T >G is associated,but eotaxin-3 +77 is not associated with asthma susceptibility,eosinophilic asthma and non-eosinophilic asthma.关键词
支气管哮喘/嗜酸性粒细胞趋化蛋白/单核苷酸多态性/易感性Key words
bronchial asthma/eotaxin/single nucleotide polymorphism/susceptibility分类
医药卫生引用本文复制引用
熊晓琦,宋新宇,曾凡军,孟丽霞,阮玉姝,李文新..eotaxin-3基因+2497、+77位点SNP单核苷酸多态性与支气管哮喘易感性及类型的关系[J].山东医药,2017,57(5):12-14,3.基金项目
湖北省教育厅科研计划项目(B2015254). （B2015254）
