山东医药2017，Vol.57Issue(5)：15-18,4.DOI:10.3969/j.issn.1002-266X.2017.05.005
miR-497-5p对人宫颈癌细胞增殖的抑制作用及机制
Inhibitory effect of miR-497-5p on proliferation of human cervical carcinoma cell
摘要
Abstract
Objective To investigate the inhibitory effect and mechanism of miR-497-5p on proliferation of human cervical cancer HeLa cells.Methods The human cervical cancer HeLa cells in the logarithmic growth phase were random-ly divided into two parts.The first part was randomly divided into 4 groups:miR-497-5p mimics NC and IKCa1-wt co-transfection group,miR-497-5p mimics and IKCa1-wt co-transfection group,miR-497-5p mimics NC and IKCa1-mut co-transfection group and miR-497-5p mimics and IKCa1-mut co-transfection group which were transfected by corresponding plasmids for 48 h.The luciferase activity of HeLa cells was detected by double luciferase reporter assay system.The second part was randomly divided into 3 groups:miR-497-5p mimics transfection group,miR-497-5p mimics NC transfection group and blank control group.Cells in each group were transfected with Lipofectamin 2000.The expression of IKCa1 mRNA was detected by qRT-PCR,IKCa1 protein expression was detected by Western blotting and the proliferation ability of HeLa cells was measured by CCK-8 assay.Results The luciferase activity of miR-497-5p mimics and IKCa1-wt co-transfection group was lower than that of the other three groups of the first part (all P <0.05),and no significant difference among other three groups (all P >0.05),which confirmed that miR-497-5p and IKCa13 '-URT had specific binding sites.The expression of IKCa1 mRNA and protein of the miR-497-5p mimics transfection group was lower than that of the other two groups of the second part (all P <0.05),and there was no significant difference between the other two groups (all P >0.05).The pro-liferation ability of the miR-497-5p mimics transfection group was lower than that of the other two groups at 24,48,72 and 96 h of culture (all P <0.05),and there was no significant difference between the other two groups (all P >0.05).Con-clusion miR-497-5p can inhibit the proliferation of cervical cancer HeLa cells by negatively regulating the expression of IKCa1 gene.关键词
子宫颈癌/微小RNA-497-5p/中电导钙激活性钾离子通道/IKCa1基因/HeLa细胞/细胞增殖Key words
cevical carcinoma/micro RNA-497-5p/intermediate-conductance-Ca2 +-activated K +channel/IKCa1 gene/HeLa cells/cell proliferation分类
医药卫生引用本文复制引用
张君,高兰阳,詹平,毛熙光..miR-497-5p对人宫颈癌细胞增殖的抑制作用及机制[J].山东医药,2017,57(5):15-18,4.基金项目
四川省卫生厅科研基金资助项目(110373) （110373）
四川省沪州市科技局科研项目(2013LZLY-J30). （2013LZLY-J30）
