山东医药2017，Vol.57Issue(5)：19-21,3.DOI:10.3969/j.issn.1002-266X.2017.05.006
胰高血糖素对胰岛 β细胞胰岛素分泌的调节作用及机制
Regulatory effect of glucagon on insulin secretion of pancreatic βcell-MIN6 cells
摘要
Abstract
Objective To explore the regulation effect and mechanism of glucagon on insulin secretion of the pancre-atic βcell-MIN6 cells.Methods The cultured MIN6 cells were transfected with ICUE3,a fluorescent biosensor DNA sensitive to cyclic adenosine monophosphate (cAMP),and then were randomly divided into three groups,respectively, which were cultivated for 400-600 s under the glucose-free,low-glucose (2.8 mmol/L glucose)and high-glucose (16.7 mmol/L glucose)conditions.The cells in each group were then randomly divided into four groups:0,100,500,1000 ng/L groups which were treated with 0,100,500,1000 ng/L glucagon for 350-600 s.Using fluorescence resonance ener-gy transfer technology (FRET)to detect the cAMP levels in MIN6 cells,and the Ins secretion was measured by enzyme-linked immune sorbent assay (ELISA).Results Under the glucose-free condition,the levels of cAMP and Ins secretion in MIN6 cells of the 1000 ng/L group were significantly higher than those in the 500 ng/L group,and significant difference was found between every two groups (all P <0.05 ).Under the low-glucose and high-glucose conditions,the levels of cAMP and Ins secretion in MIN6 cells:1000 ng/L group >500 ng/L group >100 ng/L group >0 ng/L group,signifi-cant difference was found between every two groups (all P <0.05).And under the high glucose environment,the differ-ence was more significant.Conclusion Glucagon may stimulate insulin secretion by increasing cAMP levels in the way of concentration gradient within the islet βcell line-MIN6 cells,and this trend is glucose-dependent.关键词
胰高血糖素/胰岛素/胰岛β细胞/MIN6细胞/环磷酸腺苷Key words
glucagon/insulin/pancreatic βcells/MIN6 cells/cyclic adenosine monophosphate分类
医药卫生引用本文复制引用
石艳秋,李军,李思源,张震..胰高血糖素对胰岛 β细胞胰岛素分泌的调节作用及机制[J].山东医药,2017,57(5):19-21,3.基金项目
兵团中青年科技创新领军人才项目(2015BC001) （2015BC001）
兵团科技计划立项项目(2014AB049) （2014AB049）
兵团博士资金专项(2012BB019). （2012BB019）
