山东医药2017，Vol.57Issue(6)：13-16,4.DOI:10.3969/j.issn.1002-266X.2017.06.004
荜茇酰胺对人肺腺癌 A549细胞辐射敏感性的影响
Effect of piplartine on radio-sensitization of human pulmonary adenocarcinoma A549 cells
肖楠 1徐党辉 2潘志尧 3姚志峰4
作者信息
- 1. 江苏联合职业技术学院南京卫生分院，南京210038
- 2. 南京中医药大学第一附属医院
- 3. 浙江大学医学院
- 4. 南京医科大学第二附属医院
- 折叠
摘要
Abstract
Objective To investigate the effect of piplartine on radio-sensitization of human pulmonary adenocarcino-ma A549 cells.Methods A549 cells cultured in vitro were treated with 1, 2, 4, 8 and 16 μmol/L piplartine for 24, 48 and 72 h.CCK8 assay was used to measure the proliferation inhibition of piplartine on A 549 cells in each group and then we selected the minimum concentration which had no obvious toxic effect on A 549 cells.A549 cells were divided into four groups:the control, piplartine group, radiation group and the combination treatment group .The control group was not trea-ted.The piplartine group was given the concentration of 1μmol/L piplartine , the radiation group was given X-ray radiation of 8 Gy alone, and the combination treatment group was given the concentration of 1 μmol/L piplartine for 48 h, then was exposed to X-ray radiation of 8 Gy.CCK8 assay was used to measure the proliferation inhibition of piplartine on A 549 cells in each group.Cell cycle and apoptosis of A549 cells were analyzed by flow cytometry (FCM).Results After being trea-ted with piplartine for 24, 48 and 72 h, the proliferation inhibition rate of the piplartine group was lower than that of the other groups (all P<0.05).The proliferation inhibition in the combination treatment group was higher than that of the ra -diation group and the piplartine group at 48 h (all P<0.05).The percentage of cells in the G0/G1 phase of the combina-tion treatment group was significantly increased , and was decreased in the S phase , and apoptosis rate was also higher than that of the radiation group (P<0.05).Conclusion Piplartine at the concentration of 1 μmol/L can enhance the radio-sensitivity of A549 cells, which may be associated with the enhancement of radiation-induced inhibition of cell proliferation and induction of apoptosis as well as cell arrest in G 0/G1 phase.关键词
荜茇酰胺/肺腺癌/X射线/辐射敏感性/细胞增殖/细胞周期/细胞凋亡Key words
piplartine/pulmonary adenocarcinoma/X-ray/radio-sensitization/cell proliferation/cell cycle/apopto-sis分类
医药卫生引用本文复制引用
肖楠,徐党辉,潘志尧,姚志峰..荜茇酰胺对人肺腺癌 A549细胞辐射敏感性的影响[J].山东医药,2017,57(6):13-16,4.
