水生生物学报2017，Vol.41Issue(2)：285-295,11.DOI:10.7541/2017.35
尖毛虫属ActinⅠ、α-TBP和DNApolα乱序基因的模式研究
EVOLUTION OF THE SCRAMBLED PATTERN OF THEACTINⅠ,A-TBP AND DNA POL A GENE WITHIN THE GENUS OXYTRICHA (PROTOZOA, CILIATES)
摘要
Abstract
Genes in the germline (micronuclear) genome of protozoan ciliates are interrupted by multiple, non-coding sequences called internal eliminated segments (IESs) and macronuclear destined segments (MDSs). In the micronuc-leus, the MDSs are not arranged sequentially but scrambled for some genes. Studying scrambled gene structures will provide a better understanding of the molecular mechanisms of its evolution. In this study, we compared the complete macronuclear gene sequences and complete/incomplete micronuclear gene sequences of Actin I, α-telomere-binding protein (α-TBP), and DNA polymerase α (DNApol α) of Oxytricha species (Oxytricha sp. (ZJ)) collected from water samples of Huguang mangrove in Zhanjiang with those of other Oxytricha species, and had 3 major findings. The scrambled patterns of the micronuclearActin I gene in O. sp. (ZJ) were similar with those of O. nova, but shown signi-ficant differences with other species in genus Oxytricha. We revealed that the shifts of MDS-IES junctions between scrambled MDSs are not more conservative than those between non-scrambled MDSs, which is inconsistent with a pre-vious study. The scrambled pattern of micronuclearα-TBP gene in O. sp. (ZJ) is same with that of O. nova, and the lengths of their IESs of these two species are also similar. The scrambled pattern of micronuclear DNA pol α gene inO. sp. (ZJ) is different from any of the previously reported species, and it is only somewhat similar to O. trifallax. Moreover, an IES transited to MDS was observed in DNA pol α gene, which caused the missing of its original MDS.关键词
尖毛虫/乱序基因/大核命运序列/内部删除序列/肌动蛋白Ⅰ型/端粒结合蛋白/DNA聚合酶αKey words
Oxytricha/Scramble gene/Macronuclear destined sequences (MDSs)/Internal eliminated sequences (IESs)/ActinⅠ/α-telomere-binding protein (α-TBP)/DNA polymerase α (DNA pol α)分类
生物科学引用本文复制引用
杨然,陈天兵,黄俊,石文俊,区淑青,伊珍珍..尖毛虫属ActinⅠ、α-TBP和DNApolα乱序基因的模式研究[J].水生生物学报,2017,41(2):285-295,11.基金项目
国家自然科学基金面上项目(31471973) （31471973）
广东省特支计划百千万工程青年拔尖人才项目 （）
2015年大学生创新创业训练计划华南师范大学校级项目资助 [Supported by the National Natural Science Foundation of China (31471973) （31471973）
Special Support Program of Guangdong Province （）
2015 SCNU Undergraduate Training Program for Innovation and Entrepreneurship] （）
