水生生物学报2017，Vol.41Issue(2)：296-305,10.DOI:10.7541/2017.36
日本鳗鲡TLR21基因的鉴定、免疫应答与启动子分析
MOLECULAR CLONING AND CHARACTERIZATION OF TLR21 GENE FROM JAPANESE EEL, ANGUILLA JAPONICA
摘要
Abstract
Toll like receptor 21 (TLR21) is a non-mammalian Toll-like receptor that recognizes unmethylated CpG DNA to trigger innate immune responses against pathogenic microorganism infection. The present study cloned the full-length cDNA sequence of TLR21 (AjTLR21) from Japanese eel, Anguilla japonica, which had the common charac-teristics of Toll like receptors (TLRs). The intronless AjTLR21 shared similar gene structure with other vertebrate TLR21 genes. AjTLR21 widely expressed in all examined tissues/organs with the highest expression in blood. Poly I:C treatment significantly induced the expression of AjTLR21 in spleen and kidney at 8 hours and in blood, gill, intestine and spleen at 16 hours (P<0.05). Luciferase reporter assay exhibited that the 5′ flanking region of the AjTLR21 contains positive regulatory elements for Poly I:C induced AjTLR21 expression. The stimulation of Edwardsiella tarda also in-creased the expression of AjTLR21 in blood and kidney at both 16h and 72h, suggesting an important role in anti-bac-terium immune response. Our results revealed multiple effects of teleosts TLR21 in immune system, which has impor-tant theoretical significance and application value to understand the immunological function of AjTLR21.关键词
日本鳗鲡/TLR21/基因表达/免疫应答/启动子Key words
Anguilla japonica/TLR21/Expression/Immune response/Promoter分类
生物科学引用本文复制引用
李春艳,黄贝,熊静,彭喜霞,梁英,聂品,黄文树..日本鳗鲡TLR21基因的鉴定、免疫应答与启动子分析[J].水生生物学报,2017,41(2):296-305,10.基金项目
福建省自然科学基金(2014J05042、2012J06008) （2014J05042、2012J06008）
国家自然科学基金(31402329、31174238和U1205123)资助 [Supported by the National Natural Science Foundation of Fujian Province (2014J05042, 2012J06008) （31402329、31174238和U1205123）
the National Natural Science Foundation of China (31402329, 31174238 and U1205123)] （31402329, 31174238 and U1205123）
