水生生物学报2017，Vol.41Issue(2)：321-325,5.DOI:10.7541/2017.39
应用DNA分子标记鉴定黄颡鱼、瓦氏黄颡鱼及其杂交种的研究
IDENTIFICATION OF PELTEOBAGRUS FULVIDRACO, PELTEOBAGGRUS VACHELLI AND THEIR INTERSPECIFIC HYBRID BY DNA MARKERS
摘要
Abstract
Yellow catfish (Pelteobagrus fulvidraco) is an important aquacultural fish species in China. Since hydrid breeding is an efficient method to improve aquaculture products, interspecific hybrid between Pelteobagrus fulvidraco and its close species Pelteobaggrus vachelli that grows faster than Pelteobagrus fulvidraco, has been massively pro-duced. However, it is hard to distinguish Pelteobagrus fulvidraco from the interspecific hybrid by morphological fea-tures. Moreover, the interspecific hybrid could not being used as parents for the existence of sterile hybrids. Previously, we constructed a bacterial artificial chromosome (BAC) library from YY super-male yellow catfish and screened out a BAC clone containing the sex-linked marker, Pf62-Y. In this study, we identified a novel gene inad-like in Pelteoba-grus fulvidraco after sequencing the BAC clone, and the exons of inad-like are the same in X and Y chromosomes. Moreover, we cloned partial intron sequences of inad-like in Pelteobaggrus vachelli based on the exon sequences of inad-like in Pelteobagrus fulvidraco. Interestingly, there is a small segment deletion in Pelteobaggrus vachelli in compa-rison with the corresponding sequence in Pelteobagrus fulvidraco. Based on the significant genetic difference between Pelteobagrus fulvidraco and Pelteobaggrus vachelli sequences, we designed one pair of primers, by which 1908 bp and 1178 bp were amplified in Pelteobagrus fulvidraco and Pelteobaggrus vachelli, respectively. Moreover, both 1908 bp and 1178 bp were amplified in the interspecific hybrid. Together, this new genetic marker develops a highly stable and efficient method for distinguishing Pelteobagrus fulvidraco and its interspecific hybrid.关键词
黄颡鱼/瓦氏黄颡鱼/杂交黄颡鱼/分子标记/种类鉴定Key words
Pelteobagrus fulvidraco/Pelteobaggrus vachelli/Hybrid/Molecular marker/Species identification分类
生物科学引用本文复制引用
巩高瑞,张晋,丹成,桂建芳,梅洁..应用DNA分子标记鉴定黄颡鱼、瓦氏黄颡鱼及其杂交种的研究[J].水生生物学报,2017,41(2):321-325,5.基金项目
淡水生态与生物技术国家重点实验室自主课题(2015FB03)资助 [Supported by Fund of State Key Laboratory of Freshwater Ecology and Biotechnology] （2015FB03）
