水生生物学报2017，Vol.41Issue(2)：326-333,8.DOI:10.7541/2017.40
五个红罗非鱼群体的遗传多样性分析
GENETIC DIVERSITY ANALYSIS IN FIVE RED TILAPIA POPULATIONS
摘要
Abstract
The genetic diversity and phylogenetic relationships among five breed varieties of red tilapia (GD, ZZ, FL, MY, ML) were analyzed by using 22 pairs of microsatellite primers. Stable and clear amplification bands could be amplified in 16 pairs of primers from 5 red tilapia populations by PCR. 16 pairs of microsatellite DNA primers which were selected and proved to amplify successfully on red tilapia by pre-experiment were used. The genetic diversity in-dex of 5 red tilapia populations showed that: a total of 146 alleles were detected from 16 microsatellite markers. The ave-rage allele numbers ranged from 6.5625 to 8.5625 and the average effective allele numbers ranged from 4.1495 to 6.1330, respectively. The expected value of average heterozygosities ranged from 0.7491 to 0.8247 and the average polymorphism information contents were ranged from 0.6939 to 0.7840, respectively. Chi-square tests showed that most loci in the 5 red tilapia groups deviated from Hardy-Weinberg equilibrium. The largest genetic distance (0.4827) and the lowest genetic similarity index (0.6171) were found between red tilapia (GD) and red tilapia (ZZ), which sug-gested a farthest phylogenetic relationship between these two groups. On the other hand, the highest genetic similarity index (0.9069) and the lowest genetic distance (0.0977) were found between red tilapia (FL) and red tilapia (ML), which indicated a closer phylogenetic relationship between these two populations. Phylogenetic relationship of the five populations was analyzed by using UPGMA methods and the results showed that red tilapia (FL) and red tilapia (ML) populations were clustered in the first group, the red tilapia (ZZ) in the second group, the red tilapia (MY) in the third group, while the red tilapia (GD) was in a separate group. Such results indicated that the five red tilapia populations maintained relatively high genetic diversity and still had potential for breeding.关键词
红罗非鱼/微卫星DNA/遗传多样性/遗传关系Key words
Red tilapia/Microsatellite DNA/Genetic diversity/Genetic relationship分类
生物科学引用本文复制引用
何金钊,陈诏,陈子桂,徐鸿飞,赵何勇,吕业坚..五个红罗非鱼群体的遗传多样性分析[J].水生生物学报,2017,41(2):326-333,8.基金项目
广西科技计划项目(桂科AB16380029) （桂科AB16380029）
广西科学研究与技术开发计划项目(桂科能14121008-1-6) （桂科能14121008-1-6）
广西水产畜牧兽医局渔业生产项目[桂渔牧财(2013)35、桂渔牧财(2014)52、桂渔牧财(2015)21] （2013）
广西水产遗传育种与健康养殖重点实验室开放课题(GXKL-AQUA-2014-07)资助 [Supported by the Science and Technology Projects in Guangxi Zhuang Autonomous Region (GKAB16380029) （GXKL-AQUA-2014-07）
the Scientific research and Technological Development Projects in Guangxi Zhuang Autonomous Region (GKN14121008-1-6) （GKN14121008-1-6）
Fisheries Production Project of Aquatic Animal Husbandry and Veterinary Bureau of Guangxi Zhuang Autonomous Region [GYMC(2013)35, GYMC(2014)52, GYMC(2015)21] （2013）
Open Fund of Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture (GXKL-AQUA-2014-07)] （GXKL-AQUA-2014-07）
