水生生物学报2017，Vol.41Issue(2)：334-345,12.DOI:10.7541/2017.41
基于第二代测序技术兰州鲇线粒体基因组全序列测定与分析
SEQUENCING AND ANALYSIS OF THE COMPLETE MITOCHONDRIAL GENOME OF SILURUS LANZHOUENSIS BASED ON NEXT GENERATION SEQUENCING TECHNOLOGIES
摘要
Abstract
In this study, the high-throughput next-generation sequencing technology was used to build Lanzhou catfish (Silurus lanzhouensis) mitochondrial genome sequence, and the complete sequence and characteristics for mitochond-rial genome has been analyzed. The main results were demonstrated in detail. The circular genome was 16523 bp in length with the typical mtDNA components, and the base composition showed a tendency of high A+T and low G+C. Two promoters (ATG, GTG) and three terminators (TAG, TAA and T or TA) were found in all 13 PCGs. Except tRNA-Ser(AGN) gene showed the shortage of DHU, the other 21 genes of tRNA could be folded in clover shape. The 45 stem-loop structures and 4 structural domains were identified in secondary structure of 12S rRNA, and 54 stem-loop struc-tures and 6 structural domains were identified in 16S rRNA. Three different structural domains of termination se-quence region (TAS1, TAS2), central conservative region (CSB-F, CSB-E and CSB-D) and conservative region (CSB1, CSB2 and CSB3) were divided in the controlled region that remains key sequence label. The non-code region has a conserva-tive sequence to control light chain starting copy. The complete sequence of mitochondrial genome and general COX1 label could be used for the study of evolutionary relationship between S. lanzhouensis and other Siluriformes species.关键词
兰州鲇/线粒体基因组/结构特征/系统发育Key words
Silurus lanzhouensis/Mitochondrial genome/Structure and characteristics/Phylogeny分类
生物科学引用本文复制引用
连总强,滚双宝,李力,张锋,肖伟,吴旭东..基于第二代测序技术兰州鲇线粒体基因组全序列测定与分析[J].水生生物学报,2017,41(2):334-345,12.基金项目
国家自然科学基金项目(31360633) （31360633）
国家大宗淡水鱼产业技术体系项目(CARS-46-54) （CARS-46-54）
宁夏对外科技合作项目资助 [Supported by the National Natural Science Foundation of China (31360633) （31360633）
National Technology System for Conventional Freshwater Fish Industries (CAR-46-54) （CAR-46-54）
the External Cooperation Projects of Ningxia, China] （）
