水生生物学报2017，Vol.41Issue(2)：346-355,10.DOI:10.7541/2017.42
卵巢成熟期和退化期星斑川鲽脑垂体和下丘脑组织的转录组比较分析
COMPARISON ANALYSIS ON TRANSCRIPTOME IN THE PITUITARY GLAND AND HYPOTHALAMUS OF STARRY FLOUNDER (PLATICHTHYS STELLATUS) AT THE OVARY MATURATION AND DEGRADATION STAGES
摘要
Abstract
To investigate the relationship of genes expression between brain tissue and ovary development, and explore more functional genes of Platichthys stellatus, total RNA from the mixed sample of pituitary gland and hypothalamus at the ovary maturation and degradation stages were isolated and sequenced by HiSeq 2000 high throughput sequen-cing technology. After assembly and splicing, 24128 unigenes from a total of 30640 unigenes were annotated based on the blast homology alignment. All 29137 unigenes with eggNog annotations were divided into 26 categories that were involved in physiological processes, such as signaling transduction, translation mechanism, etc. The results from KEGG pathway analysis showed that all unigenes at the ovary maturation stage were involved in metabolic pathways with 135 up-regulated sequences, while the unigenes at the ovary degradation stage were associated with 192 metabolic path-ways with 648 up-regulated sequences. Moreover, 334 differentially expressed genes (DEGs) were up-regulated at the ovary maturation stage, while 987 up-regulated DEGs were observed at the ovary degradation stage. Total 408 uni-genes were involved in reproductive and endocrine regulation, and 1508 unigenes were involved in signaling mo-lecules. The expression of some reproduction-associated DEGs including growth hormone releasing hormone (GnRH), neurokinin B (NKB), gonadotropin (GtH), follicle stimulating hormone (FSH), kisspeptin (Kiss), and prolactin-relea-sing peptide receptor (PrRPR) were verified by qRT-PCR. All genes except FSH were consistent with the results of the transcriptome sequencing.关键词
星斑川鲽/脑组织/转录组测序/实时定量PCRKey words
Platichthys stellatus/Brain tissue/Transcriptome sequencing/qRT-PCR分类
农业科技引用本文复制引用
周江,王丽娟,王波,郑凤荣,王长海..卵巢成熟期和退化期星斑川鲽脑垂体和下丘脑组织的转录组比较分析[J].水生生物学报,2017,41(2):346-355,10.基金项目
国家高技术发展计划"863项目"(2012AA10A413、2012AA10A408) （2012AA10A413、2012AA10A408）
国家海洋公益性行业科研专项(201305005)资助[Supported by the National High-tech R&D Program "863 Program" (2012AA10A413, 2012AA10A408) （201305005）
Public Science and Technology Research Funds Projects of Ocean (201305005)] （201305005）
