生物技术通报2017，Vol.33Issue(3)：58-65,8.DOI:10.13560/j.cnki.biotech.bull.1985.2017.03.009
CRISPR/Cas基因编辑技术及其在真菌中的应用
CRISPR/Cas Genome Editing Technology and Its Application in Fungi
摘要
Abstract
CRISPR/Cas system is a self-defense system against exogenous virus or plasmid in bacteria and archaea. CRISPR/Cas9,an expeditious genome editing technology,was developed according to the type II CRISPR/Cas system. Nowadays,the CRISPR/Cas9 genome editing technology has broadly utilized in gene function and genetic modification research in animal,plant and microorganism. Here,we briefly introduce the structure,mechanism and classification of CRISPR/Cas system,describe the application of CRISPR/Cas9 genome editing technology in fungi,and analyze the advantages,disadvantages as well as the prospect and application values of this technology. It is aimed at providing a useful reference for researchers in genetic modification of fungi.关键词
真菌/基因组编辑技术/CRISPR/Cas9/基因功能Key words
fungi/genome editing technology/CRISPR/Cas9/gene function引用本文复制引用
殷朝敏,范秀芝,史徳芳,高虹..CRISPR/Cas基因编辑技术及其在真菌中的应用[J].生物技术通报,2017,33(3):58-65,8.基金项目
湖北省农业科学院竞争性科技计划项目(2016jzxjh016) （2016jzxjh016）
