生物技术通报2017，Vol.33Issue(3)：175-179,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017.03.025
miR-124靶向TNF-α 促进猪皮下脂肪细胞分化
miR-124 Regulates Porcine Adipocyte Differentiation Trough Targeting TNF-α
摘要
Abstract
In the previous research,porcine TNF-α was found to be the target of miR-124 by dual luciferase assay. Here we verifywhether miR-124 has the effect on the differentiation of porcine adipocyte. Porcine pre-adipocyte was transfected with miR-124 mimics orinhibitor to over-express or to repress miR-124,and then induced into mature adipocytes. The accumulation of lipid droplets was observedusing Oil Red O staining and the amount of glycerol and triglycerides(TG)were detected by TG assay and glycerol assay. The expressionsof PPARγ(proliferator-activated receptor-γ),FASN(fatty acid synthase),and HSL(hormone-sensitive lipase)were detected by realtime fluorescence quantitative PCR. The result showed that overexpression of miR-124 repressed the expression of TNF-α protein,therefore,lipid droplets were more than that in the control,TG increased significantly(P < 0.01),also the same for glycerol(P < 0.05),andthe expressions of PPARγ,FASNT,and HSL significantly up-regulated(P < 0.01). Repressing miR-124 resulted in the reduction oflipid droplets and the significant decrease of TG,and the expressions of PPARγ and FASNT significantly down-regulated(P < 0.05). Inconclusion,miR-124 might regulate the differentiation of porcine adipocyte by suppressing TNF-α,laying a foundation for future studies onthe mechanism of miR-124 regulating lipid metabolism.关键词
miR-124/猪/脂肪分化/TNF-αKey words
miR-124/porcine/adipocyte differentiation/TNF-α引用本文复制引用
李虹仪,李家标,郑艺林,吉卢琳,张茂,郑恩琴..miR-124靶向TNF-α 促进猪皮下脂肪细胞分化[J].生物技术通报,2017,33(3):175-179,5.基金项目
福建省大学生创新创业训练计划项目(201511312055),福建省自然科学基金项目(2014J05043),龙岩学院博士启动基金项目(LB2013012) （201511312055）
