中国组织工程研究2017，Vol.21Issue(6)：836-842,7.DOI:10.3969/j.issn.2095-4344.2017.06.003
生物骨组织立体培养分化和动物体内修复实验
Three-dimensional culture and differentiation of biological bone tissue and itsin vivo repair effect in an animal model
摘要
Abstract
BACKGROUND:In vitro culture of tissue-engineered bone is an important method for bone repair. Three-dimensional (3D) printed bone stents combined with bioreactor culture are of significance in bone tissue engineering. OBJECTIVE:To study thein vivo repair effect of the 3D printed biomaterial scaffold with human mesenchymal stem cells (hMSCs) cultured in bioreactor. METHODS:The scaffold was constructed by poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA)via 3D printing and freeze-dying techniques, and then hMSCs were seeded onto the scaffold and cultured in bioreactors. Al rabbits were numbered and divided into control (No.1 and 2), experimental 1 (No. 3 and 4) and experimental 2 (No. 5 and 6) groups, and each group had two subgroups positive and negative. The rabbit left distal femur in each group was modeled into bone defect and the single PLGA/HA scaffold, PLGA/HA scaffold carrying non-induced hMSCs were implanted in the positive and negative groups of the control group, respectively; the PLGA/HA-201405-1 and PLGA/HA-201405-2carrying induced hMSCs were implanted into the positive and negative subgroups of the experimental 1 and 2 groups, respectively. Additionaly, the right femur in the experimental 2 group was driled only. The osteogenesis ability and biodegradability were determined using electron microscope, thein vivorepair was observed through CT examination, and the histopathological examination was performed after bone healing. RESULTS AND CONCLUSION:The scaffold with topological structure suitable for cellseeding was prepared. A large number of new calcium nodules were observed under electron microscope in the experimental groups indicating overt achievement in bone healing. These results suggest that the prepared scaffold achieves a good repair effect preliminarily.关键词
生物材料/骨生物材料/聚乳酸-羟基乙酸/羟基磷灰石/3D打印/间充质干细胞/生物反应器/硬骨分类
医药卫生引用本文复制引用
王妍,王汉中,张英,张丽君,田建明,陈献雄..生物骨组织立体培养分化和动物体内修复实验[J].中国组织工程研究,2017,21(6):836-842,7.基金项目
广东省教育部项目(2012B091100408) （2012B091100408）
深圳市科技创新委项目(ZYA201106100083A （）
GGJS20130331152344401) （）
校级重点项目(601522k27009) the Project of Educational Department of Guangdong Province, No. 2012B091100408 （601522k27009）
the Scientific and Technologic Innovation Project of Shenzhen, No. ZYA201106100083A, GGJS20130331152344401 （）
the Key Project ofShenzhen Polytechnic, No. 601522k27009 （）
