中国组织工程研究2017，Vol.21Issue(6)：843-847,5.DOI:10.3969/j.issn.2095-4344.2017.06.004
血管基质成分联合脱细胞骨基质-壳聚糖支架修复桡骨缺损
Stromal vascular fraction combined with acellular bone matrix-chitosan scaffold for radical defect repair
摘要
Abstract
BACKGROUND:Artificial tissue-engineered bone combined with acel ular bone matrix has been shown to be favorable for bone repair. OBJECTIVE:To explore the safety and biocompatibility of the stromal vascular fraction of the adipose tissue combined with the acel ular bone matrix-chitosan scaffold in the repair of rabbit radial defects. METHODS:A total of 38 New Zealand rabbits were selected, 3 rabbits were used to extract stromal vascular fraction of adipose tissue, 3 used to prepare acel ular bone matrix and 32 divided into experimental and control groups. Models of rabbit radial defects were established using Brownlow method. The rabbits in the experimental group were treated with the SVF of adipose tissue combined with the acel ular bone matrix-chitosan scaffold, while those controls received no treatment. General situation, gross observation, X-ray examination, histological observation and Lane-Sandhu scores were performed at 2 and 4 months postoperatively. RESULTS AND CONCLUSION:No infections occurred in both two groups at 2 and 4 months postoperatively, but the activity level and degree of healing in the experimental group were significantly better than those in the control group. In the experimental group, there were high-density shadows at 2 months postoperatively and the X-ray image of the bone defect site was the same as that of the normal one at 4 months, while bone nonunion occurred in the control group. The bone tissues in the experimental group grew significantly better than that in the control group at 2 and 4 months postoperatively, and the Lane-Sandhu histological scores in the experimental group were significantly higher than those in the control group at 2 and 4 months postoperatively (P<0.05). These results indicate that the stromal vascular fraction combined with the acel ular bone matrix-chitosan scaffold exhibits safety and biocompatibility in the repair of rabbit radical defects.关键词
生物材料/骨生物材料/血管基质成分/脱细胞骨基质/壳聚糖/桡骨缺损分类
医药卫生引用本文复制引用
邵擎东,汪铮,李宇飞,许天明,孙久一,江峰,吕峰霞..血管基质成分联合脱细胞骨基质-壳聚糖支架修复桡骨缺损[J].中国组织工程研究,2017,21(6):843-847,5.基金项目
全军医药卫生科研项目(14ZD09) the Military Medical Research Project of Chinese PLA, No. 14ZD09 （14ZD09）
