中国组织工程研究2017，Vol.21Issue(6)：848-853,6.DOI:10.3969/j.issn.2095-4344.2017.06.005
植入含感觉神经束和血管束组织工程骨修复股骨大段骨缺损:降钙素基因相关肽Ⅰ型受体的中长期表达
Implantation of tissue-engineered bone with vascular bundles or sensory nerve tracts for large femoral defects:the middle-and long-term expression of calcitonin gene related peptide type Ⅰ receptor
摘要
Abstract
BACKGROUND:Previous study has proved that implantation of the tissue-engineering bone with vascular bundles or sensory nerve tracts can promote bone formation, which may be related to the expression of sensory neuropeptide receptors. OBJECTIVE:To investigate the effect of implantation of tissue-engineered bone containing vascular bundles and sensory nerve tracts on the middle-and long-term expression of calcitonin gene-related peptide type Ⅰ receptor (CGRP1R) in the repair of rabbit large femoral defects. METHODS:Thirty-six New Zealand white rabbits were enrol ed and modeled into 1.5 cm femoral defects, and then randomized into three groups. Bone marrow mesenchymal stem cells (BMSCs) were subjected to osteoblastic induction for 7 days, and then seeded onto the β-calcium phosphate scaffold to construct the tissue-engineered scaffold.In sensory nerve group, the tissue-engineered scaffold was implanted into the defect region, and autologous sensory nerve bundles were implanted into the lateral groove of the tissue-engineered bone;in vascular bundle group, the tissue-engineered scaffold was implanted, and autologous femoral blood bundles were implanted into the lateral groove of the tissue-engineered bone;in blank control group, only the tissue-engineered scaffold was implanted. X-ray examination, immunohistochemistry and real-time PCR were performed at 24 and 48 weeks postoperatively. RESULTS AND CONCLUSION:The X-ray scores in the sensory nerve and vascular bundle groups were significantly higher than that in the control group (P<0.05), but no significant difference was found between sensory nerve and vascular bundle groups. Real-time PCR found that the expression level of CGRP1R mRNA in the vascular bundle group was significantly higher than that in the other two groups (P<0.05), but showed no significant difference between sensory nerve and blank control groups.Immunohistochemistry findings showed that CGRP1R positive expression rate in the sensory nerve and vascular bundle groups was higher than that in the blank control group. These results reveal that implantation of the tissue-engineered bone containing vascular bundles can promote the CGRP1R expression.关键词
生物材料/骨生物材料/组织工程骨/感觉神经血管化/降钙素基因相关肽Ⅰ型受体/兔分类
医药卫生引用本文复制引用
陈思圆,王簕,江汕,裴国献..植入含感觉神经束和血管束组织工程骨修复股骨大段骨缺损:降钙素基因相关肽Ⅰ型受体的中长期表达[J].中国组织工程研究,2017,21(6):848-853,6.基金项目
广东省医学科研基金立项课题(B2012277) （B2012277）
湛江市科技攻关计划项目(2012C3103031) the Medical Research Foundation of Guangdong Province, No. B2012277 （2012C3103031）
the Key Technologies Research & Development Program of Zhanjiang, No. 2012C3103031 （）
