中国组织工程研究2017，Vol.21Issue(6)：854-858,5.DOI:10.3969/j.issn.2095-4344.2017.06.006
生物活性玻璃修饰聚对苯二甲酸乙二醇酯对骨愈合的影响
Bioactive glass modified polyethylene terephthalate promotes bone healing
翟昕元 1林小风2
作者信息
- 1. 三峡大学体育学院,湖北省宜昌市 443002
- 2. 长江大学医学院,湖北省荆州市 434023
- 折叠
摘要
Abstract
BACKGROUND:Polyethylene terephthalate (PEA) holds good mechanical properties, but its biocompatibility needs to be improved. OBJECTIVE:To investigate the effect of bioactive glass modified PEA on bone healing. METHODS:In vitro experiment:PEA (control group) and bioactive glass modified PEA (experimental group) were respectively co-cultured with MC3T3-E1 cells. The cell morphology was observed at 3 days of culture, and the cell proliferation and alkaline phosphatase activity were detected at 1, 3 and 5 days of culture. In vivo experiment:24 New Zealand white rabbits were enrolled, modeled into unilateral knee ligament rupture, and then randomly allotted to two groups. Bioactive glass modified PEA and PEA were implanted into experimental and control groups, respectively. Biomechanics and histological changes were compared between groups at 6 and 12 weeks after transplantation. RESULTS AND CONCLUSION:In vitro experiment:the cell density in the experimental group was significantly higher than that in the control group after 3-day culture (P<0.05);the cell proliferation and alkaline phosphatase activity were significantly higher than those in the control group at 3 and 5 days of culture (P<0.05). In vivo experiment:the maximum axial pull-out strength in the experimental group was significantly greater than that in the control group at 6 and 12 weeks after implantation (P<0.05). In the control group, the defect was obvious and there were a large number of inflammatory cells at 6 weeks after implantation;the defect region became fuzzy, abundant inflammatory cells existed and new bone formed on the surface at 12 weeks after implantation. In the experimental group, there were newly formed bones and trabeculae formed on the interface, the scar tissue became less, and the interface width was narrow at 6 weeks after implantation;numerous new tissues were observed, and only a few inflammatory cells observed in the defect region at 12 weeks after implantation. These results indicate that the modified PEA with bioactive glass possesses good biocompatibility and biomechanics, which can promote bone healing.关键词
生物材料/骨生物材料/生物活性玻璃/聚对苯二甲酸乙二酯/运动学/骨愈合/PET纤维/58-S-PET复合材料/单侧半关节移植物-骨愈合模型/细胞增殖分类
医药卫生引用本文复制引用
翟昕元,林小风..生物活性玻璃修饰聚对苯二甲酸乙二醇酯对骨愈合的影响[J].中国组织工程研究,2017,21(6):854-858,5.
