中国组织工程研究2017，Vol.21Issue(6)：940-945,6.DOI:10.3969/j.issn.2095-4344.2017.06.021
多壁碳纳米管/聚左旋乳酸复合纳米纤维支架材料与小鼠神经干细胞的生物相容性
Biocompatibility of electrospun carbon nanotubes/poly(L-lactic acid) nanofiber scaffolds with mouse neural stem cells
摘要
Abstract
BACKGROUND:Poly(L-lactic acid) (PLLA) scaffold is a kind of widely used biomaterial in tissue engineering. However, low hydrophilicity and lack of surface cell recognition site of PLLA hinder its further application. OBJECTIVE:To study the biocompatibility of multi-walled carbon nanotubes/PLLA (MWCNTs/PLLA) nanofiber scaffolds with mouse neural stem cells in vitro. METHODS:Mouse neural stem cells were isolated. Then we used electrospinning to fabricate PLLA nanofibers and modified them with multi-walled carbon nanotubes. We assessed their biocompatibility with passage 3 mouse neural stem cells in vitro. RESULTS AND CONCLUSION:Scanning electron microscope showed that the neural stem cells could survive on both scaffolds. No cytotoxic effects were detected on both scaffolds by Cell Counting Kit-8 detection. The adhesion and proliferation abilities of neural stem cells on the MWCNTs/PLLA scaffold were significantly greater than those on the PLLA scaffold. Neural stem cells were found grow well and have normal morphology on both scaffolds under scanning electron microscope and by Hoechst 33342 staining. Besides, immunofluorescence staining showed MWCNTs/PLLA could promote neural stem cells to differentiate into mature neurons and neurites grew along with the nanofiber scaffold. In conclusion, the MWCNTs/PLLA nanofiber scaffold has better properties than the PLLA for transplanted cells and provides a good growth carrier for neural stem cells to be induced to differentiate into neurons, which is expected to have a great potential of applications in nerve tissue engineering.关键词
生物材料/纳米材料/纳米纤维支架材料/静电纺丝/聚左旋乳酸纤维/多壁碳纳米管/神经干细胞/生物相容性/细胞毒性/细胞黏附性/细胞增殖/国家自然科学基金分类
医药卫生引用本文复制引用
林成楷,戎利民,刘斌..多壁碳纳米管/聚左旋乳酸复合纳米纤维支架材料与小鼠神经干细胞的生物相容性[J].中国组织工程研究,2017,21(6):940-945,6.基金项目
国家自然科学基金(31170947 , 31470949 , 81472122) （31170947 , 31470949 , 81472122）
广东省自然科学基金(S2012020011099 , S2013010016413) （S2012020011099 , S2013010016413）
广东省科技计划项目(2012B060300008) （2012B060300008）
广州市科技计划项目(2013J4100062) （2013J4100062）
教育部博士点新教师基金(20100171120088) the National Natural Science Foundation of China, No. 31170947, 31470949, 81472122 （20100171120088）
Guangdong Natural Science Foundation, No. S2012020011099, S2013010016413 （）
Guangdong Science and Technology Planning Project, No. 2012B060300008 （）
Guangzhou Science and Technology Planning Project, No. 2013J4100062 （）
New Teacher's Fund for Doctor Stations of Ministry of Education of China, No. 20100171120088 （）
